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Abstract

Immunoassay overestimates progesterone in blood, but no studies have tested whether this occurs in saliva. We measured progesterone in saliva using immunoassay and mass spectrometry. We tested the immunoassay for cross reactivity with dehydroepiandrosterone sulfate (DHEA-S) and 17α-hydroxyprogesterone (17α-OHP). Progesterone was significantly higher in immunoassay compared to mass spectrometry. Immunoassay progesterone levels increased in when incremental levels of 17α-OHP standard was added. This effect was not observed with the addition of DHEA-S. Research using salivary progesterone immunoassay techniques should be wary, particularly with individuals taking steroid supplementation or with high levels of progesterone metabolites.

Item Type:	Article
Authors/Creators:	Ney, LJ and Felmingham, KL and Nichols, D
Keywords:	progesterone, immunoassay, mass spectrometry, saliva, DHEA-S, 17a-OHP
Journal or Publication Title:	Analytical Biochemistry
Publisher:	Academic Press Inc Elsevier Science
ISSN:	0003-2697
DOI / ID Number:	https://doi.org/10.1016/j.ab.2020.113984
Copyright Information:	Copyright 2020 Elsevier Inc.
Related URLs:	Google Scholar: Nichols, D UTAS Profile: Nichols, D
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