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A simple apparatus for electrokinetic removal of sodium dodecyl sulfate from protein digests

Pandigamage, P, Wilson, R ORCID: 0000-0003-0152-4394 and Quirino, JP ORCID: 0000-0002-8275-9672 2020 , 'A simple apparatus for electrokinetic removal of sodium dodecyl sulfate from protein digests' , The Journal of Chromatography A, vol. 1628 , pp. 1-5 , doi: 10.1016/j.chroma.2020.461443.

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Abstract

Sodium dodecyl sulfate (SDS) in proteomics samples needs to be removed and estimated prior to mass spectrometry (MS)-based analysis and to avoid MS ion-source contamination. Here, we describe an organic solvent free method to remove SDS using a simple apparatus that mainly consists of an agarose gel inside a 1 mL plastic micropipette tip and a voltage power supply with electrodes. A small volume of sample (e.g., 50 μL) is loaded on top of the gel and then voltage (cathode at the sample side) is applied with an acidic solution at the other end of the micropipette tip. Within 25 min, SDS was removed (e.g., ≥99% SDS in 3.5 mM SDS) and the peptides were retained in the sample solution. The strategy was compared to the commercially available and expensive Pierce spin column for the removal of SDS and recovery of peptides from a digested bovine serum albumin sample.

Item Type: Article
Authors/Creators:Pandigamage, P and Wilson, R and Quirino, JP
Keywords: sodium dodecyl sulfate, proteomics, protein digestion, electrophoresis
Journal or Publication Title: The Journal of Chromatography A
Publisher: Elsevier Science Bv
ISSN: 0021-9673
DOI / ID Number: 10.1016/j.chroma.2020.461443
Copyright Information:

Crown Copyright 2020

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