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Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect

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Chuang, Y-F, Phipps, AJ ORCID: 0000-0003-4689-1198, Lin, F-L, Hecht, V ORCID: 0000-0002-3539-3356, Hewitt, AW ORCID: 0000-0002-5123-5999, Wang, P-Y and Liu, G-S ORCID: 0000-0003-3379-724X 2021 , 'Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect' , Cellular and Molecular Life Sciences, vol. 78, no. 6 , 2683–2708 , doi: 10.1007/s00018-020-03725-2.

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Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system provides a groundbreaking genetic technology that allows scientists to modify genes by targeting specific genomic sites. Due to the relative simplicity and versatility of the CRISPR/Cas system, it has been extensively applied in human genetic research as well as in agricultural applications, such as improving crops. Since the gene editing activity of the CRISPR/Cas system largely depends on the efficiency of introducing the system into cells or tissues, an efficient and specific delivery system is critical for applying CRISPR/Cas technology. However, there are still some hurdles remaining for the translatability of CRISPR/Cas system. In this review, we summarized the approaches used for the delivery of the CRISPR/Cas system in mammals, plants, and aquacultures. We further discussed the aspects of delivery that can be improved to elevate the potential for CRISPR/Cas translatability.

Item Type: Article
Authors/Creators:Chuang, Y-F and Phipps, AJ and Lin, F-L and Hecht, V and Hewitt, AW and Wang, P-Y and Liu, G-S
Keywords: CRISPR/Cas, gene delivery, gene editing, gene therapy
Journal or Publication Title: Cellular and Molecular Life Sciences
Publisher: Birkhauser Verlag Ag
ISSN: 1420-682X
DOI / ID Number: 10.1007/s00018-020-03725-2
Copyright Information:

© The Author(s), under exclusive licence to Springer Nature Switzerland AG part of Springer Nature 2021

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