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Precise, accurate and user-independent blood collection system for dried blood spot sample preparation

Neto, R, Gooley, A, Breadmore, MC ORCID: 0000-0001-5591-4326, Hilder, EF ORCID: 0000-0002-4444-7870 and Lapierre, F ORCID: 0000-0003-0377-6069 2018 , 'Precise, accurate and user-independent blood collection system for dried blood spot sample preparation' , Analytical and Bioanalytical Chemistry, vol. 410, no. 14 , pp. 3315-3323 , doi: 10.1007/s00216-018-0993-y.

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Abstract

An accurate and precise 3 μL blood collection and dispensing system is presented for the preparation of dried blood spot (DBS) samples. Using end-to-end glass capillaries in conjugation with pre-punched DBS pads, a blood micro collection system was developed to eliminate the haematocrit dispersion, widely associated with DBS technology, while providing better levels of accuracy and precision during sample preparation. This methodology is compared to traditional micro-volume blood collection systems, such as a pipette and a digitally controlled analytical syringe. Results showed that % of recovery for the capillary methodology was closer to 100% across the three haematocrit (HCT) levels tested and when prepared by two users (98 to 100% for capillaries, 78 to 104% for pipette and 93 to 97% for digital syringe) attesting a higher accuracy. Additionally, by taking advantage of the capillary action mechanism to collect and dispense autonomously the desired volume of blood onto the DBS pad, coefficients of variation between two individuals were significantly lower than with standard methodologies (capillaries - 0.05 to 0.77%, pipette - 12.71 to 18.53% and digital syringe - 0.72 to 1.77%). This alternate aspiration and dispensing methodology could be used by different users without compromising accuracy or precision when handling low volumes of blood during the pre-analytical steps.

Item Type: Article
Authors/Creators:Neto, R and Gooley, A and Breadmore, MC and Hilder, EF and Lapierre, F
Keywords: dried blood spot, microsampling, glass capillary, blood, analytical chemistry
Journal or Publication Title: Analytical and Bioanalytical Chemistry
Publisher: Springer-Verlag Heidelberg
ISSN: 1618-2642
DOI / ID Number: 10.1007/s00216-018-0993-y
Copyright Information:

Copyright 2018 Springer-Verlag GmbH Germany, part of Springer Nature

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