Open Access Repository

Mesenchymal plasticity of devil facial tumour cells during in vivo vaccine and immunotherapy trials

Patchett, AL ORCID: 0000-0002-8424-4680, Tovar, C ORCID: 0000-0001-9555-2489, Blackburn, NB ORCID: 0000-0002-9774-1539, Woods, GM ORCID: 0000-0001-8421-7917 and Lyons, AB ORCID: 0000-0002-8508-5853 2021 , 'Mesenchymal plasticity of devil facial tumour cells during in vivo vaccine and immunotherapy trials' , Immunology and Cell Biology , pp. 1-13 , doi: 10.1111/imcb.12451.

Full text not available from this repository.

Abstract

Immune evasion is critical to the growth and survival of cancer cells. This is especially pertinent to transmissible cancers, which evade immune detection across genetically diverse hosts. The Tasmanian devil (Sarcophilus harrisii) is threatened by the emergence of Devil Facial Tumour Disease (DFTD), comprising two transmissible cancers (DFT1 and DFT2). The development of effective prophylactic vaccines and therapies against DFTD has been restricted by an incomplete understanding of how allogeneic DFT1 and DFT2 cells maintain immune evasion upon activation of tumour-specific immune responses. In this study, we used RNA sequencing to examine tumours from three experimental DFT1 cases. Two devils received a vaccine prior to inoculation with live DFT1 cells, providing an opportunity to explore changes to DFT1 cancers under immune pressure. Analysis of DFT1 in the non-immunised devil revealed a 'myelinating Schwann cell' phenotype, reflecting both natural DFT1 cancers and the DFT1 cell line used for the experimental challenge. Comparatively, immunised devils exhibited a 'dedifferentiated mesenchymal' DFT1 phenotype. A third 'immune-enriched' phenotype, characterised by increased PDL1 and CTLA-4 expression, was detected in a DFT1 tumour that arose after immunotherapy. In response to immune pressure, mesenchymal plasticity and upregulation of immune checkpoint molecules are used by human cancers to evade immune responses. Similar mechanisms are associated with immune evasion by DFTD cancers, providing novel insights that will inform modification of DFTD vaccines. As DFT1 and DFT2 are clonal cancers transmitted across genetically distinct hosts, the Tasmanian devil provides a 'natural' disease model for more broadly exploring these immune evasion mechanisms in cancer.

Item Type: Article
Authors/Creators:Patchett, AL and Tovar, C and Blackburn, NB and Woods, GM and Lyons, AB
Keywords: Tasmanian devil, Devil Facial Tumour Disease, transmissible cancer, cancer vaccine, epithelial-to-mesenchymal transition, mesenchymal plasticity
Journal or Publication Title: Immunology and Cell Biology
Publisher: Blackwell Publishing Asia
ISSN: 0818-9641
DOI / ID Number: 10.1111/imcb.12451
Copyright Information:

Copyright 2021 Australian and New Zealand Society for Immunology, Inc.

Related URLs:
Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page
TOP