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Quantitative proteomics provides an insight into germination-related proteins in the obligate biotrophic plant pathogen Spongospora subterranea

Balotf, S, Wilson, R ORCID: 0000-0003-0152-4394, Tegg, RS ORCID: 0000-0001-7668-7927, Nichols, DS ORCID: 0000-0002-8066-3132 and Wilson, CR ORCID: 0000-0001-5434-3816 2021 , 'Quantitative proteomics provides an insight into germination-related proteins in the obligate biotrophic plant pathogen Spongospora subterranea' , Environmental Microbiology Reports , pp. 1-12 , doi: 10.1111/1758-2229.12955.

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Abstract

The soil-borne and obligate plant-associated nature of S. subterranea has hindered a detailed study of this pathogen and in particular, the regulatory pathways driving the germination of S. subterranea remain unknown. To better understand the mechanisms that control the transition from dormancy to germination, protein profiles between dormant and germination stimulant-treated resting spores were compared using label-free quantitative proteomics. Among the ~680 proteins identified 20 proteins were found to be differentially expressed during the germination of S. subterranea resting spores. Elongation factor Tu, histones (H2A and H15), proteasome and DJ-1_PfpI, involved in transcription and translation, were upregulated during the germination of resting spores. Downregulation of both actin and beta-tubulin proteins occurred in the germinating spores, indicating that the changes in the cell wall cytoskeleton may be necessary for the morphological changes during the germination of the resting spore in S. subterranea. Our findings provide new approaches for the study of these and similar recalcitrant micro-organisms provide the first insights into the basic protein components of S. subterranea spores. A better understanding of S. subterranea biology may lead to the development of novel approaches for the management of persistent soil inoculum.

Item Type: Article
Authors/Creators:Balotf, S and Wilson, R and Tegg, RS and Nichols, DS and Wilson, CR
Journal or Publication Title: Environmental Microbiology Reports
Publisher: John Wiley & Sons Ltd.
ISSN: 1758-2229
DOI / ID Number: 10.1111/1758-2229.12955
Copyright Information:

Copyright 2021 Society for Applied Microbiology and John Wiley & Sons Ltd

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