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Methods for in vitro CRISPR/CasRx-Mediated RNA Editing

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Chuang, Y-F, Wang, P-Y, Kumar, S, Lama, S, Lin, F, Lin, F-L and Liu, G ORCID: 0000-0003-3379-724X 2021 , 'Methods for in vitro CRISPR/CasRx-Mediated RNA Editing' , Frontiers in Cell and Developmental Biology , pp. 1-13 , doi: 10.3389/fcell.2021.667879.

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Abstract

Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more precise. Specifically, CasRx, a family member of the Cas13d family, has shown great therapeutic potential. Here, we describe the in vitro methods of utilizing this powerful RNA editing platform and determine the RNA editing efficiencies for CasRx with different forms of guide RNAs (also known as gRNA or sgRNA).

Item Type: Article
Authors/Creators:Chuang, Y-F and Wang, P-Y and Kumar, S and Lama, S and Lin, F and Lin, F-L and Liu, G
Keywords: CRISPR/Cas13d, CasRx, RNA editing, VEGF (Vascular endothelial growth factor), AAV (Adeno-associated virus)
Journal or Publication Title: Frontiers in Cell and Developmental Biology
Publisher: Frontiers Research Foundation
ISSN: 2296-634X
DOI / ID Number: 10.3389/fcell.2021.667879
Copyright Information:

Copyright © 2021 Chuang, Wang, Kumar, Lama, Lin and Liu. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) License. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms

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