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High-throughput screening of biofilm formation of Listeria monocytogenes on stainless steel coupons using a 96-well plate format

Gray, JA, Chandry, PS, Bowman, JP ORCID: 0000-0002-4528-9333 and Fox, EM 2021 , 'High-throughput screening of biofilm formation of Listeria monocytogenes on stainless steel coupons using a 96-well plate format', in EM Fox and H Bierne and B Stessl (eds.), Listeria Monocytogenes: Methods and Protocols , Humana Press/Springer Nature, New York, USA, pp. 115-122.

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Abstract

Listeria monocytogenes is a foodborne pathogen capable of colonizing and persisting in the food production environment (FPE). While there are a variety of factors involved in L. monocytogenes’ ability to persist in FPE, the ability to form biofilms has the potential to increase their chance of survival and long-term colonization. Understanding the mechanisms involved in L. monocytogenes ability to form biofilms may potentially help food safety managers optimize control strategies targeting it in the FPE. In this chapter, a high-throughput method to determine L. monocytogenes ability to attach and form biofilms utilizing FPE-grade stainless steel is described. This method provides fast and efficient results, facilitating scaling up to large numbers of isolates to measure their ability to form biofilms, where lower-throughput approaches can then be utilized to further characterize isolates of interest.

Item Type: Book Section
Authors/Creators:Gray, JA and Chandry, PS and Bowman, JP and Fox, EM
Keywords: biofilm formation, high-throughput, stainless steel, 96-well microtiter plate, Listeria monocytogenes, methodology
Publisher: Humana Press/Springer Nature
DOI / ID Number: 10.1007/978-1-0716-0982-8_9
Copyright Information:

Copyright 2021 Springer Science+Business Media, LLC, part of Springer Nature

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