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Interaction of preimplantation factor with the global bovine endometrial transcriptome

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Wonfor, RE, Creevey, CJ, Natoli, M, Hegarty, M, Nash, DM and Rose, MT ORCID: 0000-0001-6058-1319 2020 , 'Interaction of preimplantation factor with the global bovine endometrial transcriptome' , PLoS One, vol. 15, no. 12 , pp. 1-21 , doi: 10.1371/journal.pone.0242874.

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Abstract

Preimplantation factor (PIF) is an embryo derived peptide which exerts an immune modulatory effect on human endometrium, promoting immune tolerance to the embryo whilst maintaining the immune response to invading pathogens. While bovine embryos secrete PIF, the effect on the bovine endometrium is unknown. Maternal recognition of pregnancy is driven by an embryo-maternal cross talk, however the process differs between humans and cattle. As many embryos are lost during the early part of pregnancy in cattle, a greater knowledge of factors affecting the embryo-maternal crosstalk, such as PIF, is needed to improve fertility. Therefore, for the first time, we demonstrate the effect of synthetic PIF (sPIF) on the bovine transcriptome in an ex vivo bovine endometrial tissue culture model. Explants were cultured for 30h with sPIF (100nM) or in control media. Total RNA was analysed via RNA-sequencing. As a result of sPIF treatment, 102 genes were differentially expressed compared to the control (Padj C3. Steroid biosynthesis was the only over-represented pathway with all genes upregulated. We demonstrate that sPIF can modulate the bovine endometrial transcriptome in an immune modulatory manner, like that in the human endometrium, however, the regulation of genes was much weaker than in previous human work.

Item Type: Article
Authors/Creators:Wonfor, RE and Creevey, CJ and Natoli, M and Hegarty, M and Nash, DM and Rose, MT
Journal or Publication Title: PLoS One
Publisher: Public Library of Science
ISSN: 1932-6203
DOI / ID Number: 10.1371/journal.pone.0242874
Copyright Information:

Copyright: © 2020 Wonfor et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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