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Use of contactless conductivity detection for non-invasive characterisation of monolithic stationary-phase coatings for application in capillary ion chromatography

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Gillespie, E, Connolly, D, Macka, M, Nesterenko, PN and Paull, B 2007 , 'Use of contactless conductivity detection for non-invasive characterisation of monolithic stationary-phase coatings for application in capillary ion chromatography' , Analyst, vol. 132, no. 12 , pp. 1238-1245 , doi: 10.1039/b711350a.

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Abstract

A capacitively-coupled contactless conductivity detector (C4D) has been utilised as an oncapillary
detector within a capillary ion chromatograph, incorporating a reversed-phase
monolithic silica capillary column semi-permanently modified with a suitable ionic surfactant.
The monolithic capillary column (150 6 0.1 mm i.d.) was modified using sodium dioctyl
sulfosuccinate (DOSS), an anionic surfactant, for the separation of small inorganic and organic
cations. With the use of the on-capillary conductivity detector, the longitudinal homogeneity and
temporal stability of the coating were investigated. The approach allowed a detailed non-invasive
observation of the nature of the ion-exchange coating over time, and an example of an application
of the technique to produce a longitudinal stationary-phase charge gradient is shown. An
investigation of the basis of the measured on-capillary conductivity was carried out with a counter
ion study, clearly showing the on-capillary detection technique could also distinguish between
chemical forms of the immobilised ion exchanger. The above method was used to produce a stable
and homogeneously-modified monolithic ion-exchange capillary column, for application to the
separation of inorganic alkaline earth cations and amino acids.

Item Type: Article
Authors/Creators:Gillespie, E and Connolly, D and Macka, M and Nesterenko, PN and Paull, B
Journal or Publication Title: Analyst
Publisher: Royal Society of Chemistry
ISSN: 0003-2654
DOI / ID Number: 10.1039/b711350a
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