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Abstract

We characterised 9 strains selected from primary isolates referable to Paramoeba/Neoparamoeba
spp. Based on ultrastructural study, 5 strains isolated from fish (amoebic gill disease
[AGD]-affected Atlantic salmon and dead southern bluefin tuna), 1 strain from netting of a floating
sea cage and 3 strains isolated from invertebrates (sea urchins and crab) were assigned to the genus
Neoparamoeba Page, 1987. Phylogenetic analyses based on SSU rDNA sequences revealed affiliations
of newly introduced and previously analysed Neoparamoeba strains. Three strains from the
invertebrates and 2 out of 3 strains from gills of southern bluefin tunas were members of the N.
branchiphila clade, while the remaining, fish-isolated strains, as well as the fish cage strain, clustered
within the clade of N. pemaquidensis. These findings and previous reports point to the possibility that
N. pemaquidensis and N. branchiphila can affect both fish and invertebrates. A new potential fish
host, southern bluefin tuna, was included in the list of farmed fish endangered by N. branchiphila.
The sequence of P. eilhardi (Culture Collection of Algae and Protozoa [CCAP] strain 1560/2)
appeared in all analyses among sequences of strain representatives of Neoparamoeba species, in a
position well supported by bootstrap value, Bremer index and Bayesian posterior probability. Our
research shows that isolation of additional strains from invertebrates and further analyses of relations
between molecular data and morphological characters of the genera Paramoeba and Neoparamoeba
are required. This complexity needs to be considered when attempting to define molecular markers
for identification of Paramoeba/Neoparamoeba species in tissues of fish and invertebrates.

Item Type:	Article
Authors/Creators:	Dykova, I and Nowak, BF and Peckova, H and Fiala, I and Crosbie, PBB and Dvorakova, H
Keywords:	Neoparamoeba strains · Paramoeba eilhardi · Phylogeny · Invertebrate infections
Journal or Publication Title:	Diseases of Aquatic Organisms
Publisher:	Inter-Research
ISSN:	0177-5103
Additional Information:	Copyright © 2007 Inter Research
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