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Dysbiosis triggers ACF development in genetically predisposed subjects


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De Santis, S, Liso, M, Vacca, M, Verna, G, Cavalcanti, E, Coletta, S, Calabrese, FM, Eri, R ORCID: 0000-0003-1688-8043, Lippolis, A, Armentano, R, Mastronardi, M, De Angelis, M and Chieppa, M 2021 , 'Dysbiosis triggers ACF development in genetically predisposed subjects' , Cancers, vol. 13, no. 2 , pp. 1-21 , doi: 10.3390/cancers13020283.

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Background: Colorectal cancer (CRC) is the third most common cancer worldwide, characterized by a multifactorial etiology including genetics, lifestyle, and environmental factors including microbiota composition. To address the role of microbial modulation in CRC, we used our recently established mouse model (the Winnie-APCMin/+) combining inflammation and genetics.Methods: Gut microbiota profiling was performed on 8-week-old Winnie-APCMin/+ mice and their littermates by 16S rDNA gene amplicon sequencing. Moreover, to study the impact of dysbiosis induced by the mother's genetics in ACF development, the large intestines of APCMin/+ mice born from wild type mice were investigated by histological analysis at 8 weeks.Results: ACF development in 8-week-old Winnie-APCMin/+mice was triggered by dysbiosis. Specifically, the onset of ACF in genetically predisposed mice may result from dysbiotic signatures in the gastrointestinal tract of the breeders. Additionally, fecal transplant from Winnie donors to APCMin/+ hosts leads to an increased rate of ACF development.Conclusions: The characterization of microbiota profiling supporting CRC development in genetically predisposed mice could help to design therapeutic strategies to prevent dysbiosis. The application of these strategies in mothers during pregnancy and lactation could also reduce the CRC risk in the offspring.

Item Type: Article
Authors/Creators:De Santis, S and Liso, M and Vacca, M and Verna, G and Cavalcanti, E and Coletta, S and Calabrese, FM and Eri, R and Lippolis, A and Armentano, R and Mastronardi, M and De Angelis, M and Chieppa, M
Keywords: colorectal cancer, microbiota, dysbiosis, ulcerative colitis, murine model
Journal or Publication Title: Cancers
Publisher: MDPI
ISSN: 2072-6694
DOI / ID Number: 10.3390/cancers13020283
Copyright Information:

Copyright: © 2021 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) license (

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