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Expression and prognostic value of MCM family genes in osteosarcoma

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Zhou, J, Wang, M, Zhou, Z ORCID: 0000-0002-0835-8686, Wang, W, Duan, J and Wu, G 2021 , 'Expression and prognostic value of MCM family genes in osteosarcoma' , Frontiers in Molecular Biosciences, vol. 8 , pp. 1-13 , doi: 10.3389/fmolb.2021.668402.

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Abstract

We performed a detailed cancer VS normal analysis to explore the expression and prognostic value of minichromosome maintenance (MCM) proteinsin human sarcoma. The mRNA expression levels of the MCM family genes in sarcoma were analyzed using data from ONCOMINE, GEPIA and CCLE databases. KEGG database was used to analyze the function of MCM2-7 complex in DNA replication and cell cycle. QRT-PCR and western blot were used to confirm the differential expression of key MCMs in osteosarcoma cell lines. Cell Counting Kit-8 and flow cytometry method were used to detect the cell proliferation and apoptosis of hFOB1.19 cells. The results showed that MCM1-7 and MCM10 were all upregulated in sarcoma in ONCOMINE database. MCM2, and MCM4-7 were highly expressed in sarcoma in GEPIA database. Moreover, all these ten factors were highly expressed in sarcoma cell lines. Furthermore, we analyzed the prognostic value of MCMs for sarcoma in GEPIA and found that MCM2, MCM3, MCM4, and MCM10 are prognostic biomarkers for human sarcoma. Analysis results using KEGG datasets showed that MCM4 and MCM6-7 constituted a core structure of MCM2-7 hexamers. We found that AzadC treatment and overexpression of MCM4 significantly promoted hFOB1.19 cell proliferation and inhibited apoptosis. The present study implied that MCM2-4 and 10 are potential biomarkers for the prognosis of sarcoma. The prognostic role of MCM4 may be attributable to the change in its DNA methylation patterns.

Item Type: Article
Authors/Creators:Zhou, J and Wang, M and Zhou, Z and Wang, W and Duan, J and Wu, G
Keywords: DNA methylation, MCMs, expression, prognosis, sarcoma
Journal or Publication Title: Frontiers in Molecular Biosciences
Publisher: Frontiers Media S.A.
ISSN: 2296-889X
DOI / ID Number: 10.3389/fmolb.2021.668402
Copyright Information:

Copyright © 2021 Zhou,Wang, Zhou, Wang, Duan and Wu. This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License, (https://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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