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Microsatellites retain phylogenetic signals across genera in eucalypts (Myrtaceae)

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Ochieng, JW and Steane, DA and Ladiges, PY and Baverstock, PR and Henry, RJ and Shepherd, M (2007) Microsatellites retain phylogenetic signals across genera in eucalypts (Myrtaceae). Genetics and Molecular Biology, 30 (4). pp. 1125-1134. ISSN 1415-4757

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Abstract

The utility of microsatellites (SSRs) in reconstructing phylogenies is largely confined to studies below the genus
level, due to the potential of homoplasy resulting from allele size range constraints and poor SSR transferability
among divergent taxa. The eucalypt genus Corymbia has been shown to be monophyletic using morphological characters,
however, analyses of intergenic spacer sequences have resulted in contradictory hypotheses- showing the
genus as either equivocal or paraphyletic. To assess SSR utility in higher order phylogeny in the family Myrtaceae,
phylogenetic relationships of the bloodwood eucalypts Corymbia and related genera were investigated using eight
polymorphic SSRs. Repeat size variation using the average square and Nei’s distance were congruent and showed
Corymbia to be a monophyletic group, supporting morphological characters and a recent combination of the internal
and external transcribed spacers dataset. SSRs are selectively neutral and provide data at multiple genomic regions,
thus may explain why SSRs retained informative phylogenetic signals despite deep divergences. We show that
where the problems of size-range constraints, high mutation rates and size homoplasy are addressed, SSRs might
resolve problematic phylogenies of taxa that have diverged for as long as three million generations or 30 million
years.
Key words

Item Type: Article
Keywords: microsatellite phylogeny, paraphyletic, homoplasy, incongruence, eucalypts.
Journal or Publication Title: Genetics and Molecular Biology
Publisher: Sociedade Brasileira de Genetica
Page Range: pp. 1125-1134
ISSN: 1415-4757
Date Deposited: 07 Apr 2008 14:07
Last Modified: 18 Nov 2014 03:33
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