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A simple approach to induce experimental autoimmune neuritis in C57BL/6 mice for functional and neuropathological assessments

Gonsalvez, DG, Fletcher, JL ORCID: 0000-0003-2009-0798, Yoo, SW, Wood, RJ, Murray, SS and Xiao, J 2017 , 'A simple approach to induce experimental autoimmune neuritis in C57BL/6 mice for functional and neuropathological assessments' , Journal of Visualized Experiments, vol. 9, no. 29 , pp. 1-6 , doi: 10.3791/56455.

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Abstract

Experimental autoimmune neuritis (EAN) is a well-appreciated experimental model of autoimmune peripheral demyelinating diseases. EAN disease is induced by immunizing mice with neurogenic peptides to direct an inflammatory attack toward components of the peripheral nervous system (PNS). Recent advances have enabled the induction of EAN in the relatively resistant C57BL/6 mouse line using myelin protein zero (P0)106-125 or P0180-199 peptides delivered in adjuvant combined with the injection of pertussis toxin. The ability to induce EAN in the C57BL/6 strain allows for the use of the numerous genetic tools that exist on this mouse background, and thus allows the sophisticated study of disease pathogenesis and interrogation of the mechanistic action of novel therapeutics in combination with transgenic approaches. In this study, we demonstrate a simple approach to successfully induce EAN using the P0180-199 peptide in C57BL/6 mice. We also outline a protocol for the assessment of functional deficits that occur in this model, accompanied by an array of neuropathological features. Thus, this model is a powerful experimental model to study the pathogenesis of human peripheral demyelinating neuropathies, and to determine the efficacy of potential therapies that aim to promote myelin repair and protect against nerve damage in autoimmune neuritis.

Item Type: Article
Authors/Creators:Gonsalvez, DG and Fletcher, JL and Yoo, SW and Wood, RJ and Murray, SS and Xiao, J
Keywords: peripheral nervous system, demyelinating disease, animal model
Journal or Publication Title: Journal of Visualized Experiments
Publisher: MYJoVE Corporation
ISSN: 1940-087X
DOI / ID Number: 10.3791/56455
Copyright Information:

Copyright 2017 Journal of Visualized Experiments

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