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Profiling the glycome of Cardicola forsteri, a blood fluke parasitic to bluefin tuna

Coff, L, Abrahams, JL, Collett, S, Power, C, Nowak, BF ORCID: 0000-0002-0347-643X, Kolarich, D, Bott, NJ and Ramsland, PA 2021 , 'Profiling the glycome of Cardicola forsteri, a blood fluke parasitic to bluefin tuna' , International Journal for Parasitology , pp. 1-12 , doi: 10.1016/j.ijpara.2021.06.004.

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Abstract

Infections by blood flukes (Cardicola spp.) are considered the most significant health issue for ranched bluefin tuna, a major aquaculture industry in Japan and Australia. The host–parasite interfaces of trematodes, namely their teguments, are particularly rich in carbohydrates, which function both in evasion and modulation of the host immune system, while some are primary antigenic targets. In this study, histochemistry and mass spectrometry techniques were used to profile the glycans of Cardicola forsteri. Fluorescent lectin staining of adult flukes indicates the presence of oligomannose (Concanavalin A-reactive) and fucosylated (Pisum sativum agglutinin-reactive) N-glycans. Additionally, reactivity of succinylated wheat germ agglutinin (s-WGA) was localised to several internal organs of the digestive and monoecious reproductive systems. Glycan structures were further investigated with tandem mass spectrometry, which revealed structures indicated by lectin reactivity. While O-glycans from these adult specimens were not detectable by mass spectrometry, several oligomannose, paucimannosidic, and complex-type N-glycans were identified, including some carrying hexuronic acid and many carrying core xylose. This is, to our knowledge, the first glycomic characterisation of a marine platyhelminth, with broader implications for research into other trematodes.

Item Type: Article
Authors/Creators:Coff, L and Abrahams, JL and Collett, S and Power, C and Nowak, BF and Kolarich, D and Bott, NJ and Ramsland, PA
Keywords: glycan, mass spectrometry, lectin, trematode, aquaculture, xylose
Journal or Publication Title: International Journal for Parasitology
Publisher: Pergamon-Elsevier Science Ltd
ISSN: 0020-7519
DOI / ID Number: 10.1016/j.ijpara.2021.06.004
Copyright Information:

Copyright 2021 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved

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