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Genetic and gene expression analysis of flowering time regulation by light quality in lentil

Yuan, HY, Caron, CT, Ramsay, L, Fratini, R, Perez de la Vega, M, Vandenberg, A, Weller, JL ORCID: 0000-0003-2423-8286 and Bett, KE 2021 , 'Genetic and gene expression analysis of flowering time regulation by light quality in lentil' , Annals of Botany, vol. 128, no. 4 , pp. 481-495 , doi: 10.1093/aob/mcab083.

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Background and AimsFlowering time is important due to its roles in plant adaptation to different environments and subsequent formation of crop yield. Changes in light quality affect a range of developmental processes including flowering time, but little is known about light quality-induced flowering time control in lentil. This study aims to investigate the genetic basis for differences in flowering response to light quality in lentil.MethodsWe explored variation in flowering time caused by changes in red/far-red-related light quality environments of a lentil interspecific recombinant inbred line (RIL) population developed from a cross between Lens culinaris cv. Lupa and L. orientalis accession BGE 016880. A genetic linkage map was constructed and then used for identifying quantitative trait loci (QTLs) associated with flowering time regulation under different light quality environments. Differential gene expression analysis through transcriptomic study and RT-qPCR were used to identify potential candidate genes.Key ResultsQTL mapping located 13 QTLs controlling flower time under different light quality environments, with phenotypic variance explained ranging from 1.7 to 62.9 %. Transcriptomic profiling and gene expression analysis for both parents of this interspecific RIL population identified flowering-related genes showing environment–specific differential expression (flowering DEGs). One of these, a member of the florigen gene family FTa1 (LcFTa1), was located close to three major QTLs. Furthermore, gene expression results suggested that two other florigen genes (LcFTb1 and LcFTb2), MADS-box transcription factors such as LcAGL6/13d, LcSVPb, LcSOC1b and LcFULb, as well as bHLH transcription factor LcPIF6 and Gibberellin 20 oxidase LcGA20oxC,G may also be involved in the light quality response.ConclusionsOur results show that a major component of flowering time sensitivity to light quality is tightly linked to LcFTa1 and associated with changes in its expression. This work provides a foundation for crop improvement of lentil with better adaptation to variable light environments.

Item Type: Article
Authors/Creators:Yuan, HY and Caron, CT and Ramsay, L and Fratini, R and Perez de la Vega, M and Vandenberg, A and Weller, JL and Bett, KE
Keywords: flowering time, light quality, R/FR, QTL analysis, RNAseq, transcriptome, differential gene expression, Lens culinaris, Lens orientalis
Journal or Publication Title: Annals of Botany
Publisher: Oxford University Press
ISSN: 1095-8290
DOI / ID Number: 10.1093/aob/mcab083
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Copyright 2021 the Authors

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