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Dysfunctional gut microbiome networks in childhood IgE-mediated food allergy

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Lee, KH, Guo, J, Song, Y ORCID: 0000-0002-8178-5641, Ariff, A, O'Sullivan, M, Hales, B, Mullins, BJ and Zhang, G 2021 , 'Dysfunctional gut microbiome networks in childhood IgE-mediated food allergy' , International Journal of Molecular Sciences, vol. 22, no. 4 , pp. 1-10 , doi: 10.3390/ijms22042079.

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Abstract

The development of food allergy has been reported to be related with the changes in the gutmicrobiome, however the specific microbe associated with the pathogenesis of food allergy remainselusive. This study aimed to comprehensively characterize the gut microbiome and identify individual or group gut microbes relating to food-allergy using 16S rRNA gene sequencing with networkanalysis. Faecal samples were collected from children with IgE-mediated food allergies (n = 33) andwithout food allergy (n = 27). Gut microbiome was profiled by 16S rRNA gene sequencing. OTUsobtained from 16S rRNA gene sequencing were then used to construct a co-abundance network usingWeighted Gene Co-expression Network Analysis (WGCNA) and mapped onto Kyoto Encyclopediaof Genes and Genomes (KEGG) pathways. We identified a co-abundance network module to bepositively correlated with IgE-mediated food allergy and this module was characterized by a hubtaxon, namely Ruminococcaceae UCG-002 (phylum Firmicutes). Functional pathway analysis of all thegut microbiome showed enrichment of methane metabolism and glycerolipid metabolism in the gutmicrobiome of food-allergic children and enrichment of ubiquinone and other terpenoid-quinonebiosynthesis in the gut microbiome of non-food allergic children. We concluded that RuminococcaceaeUCG-002 may play determinant roles in gut microbial community structure and function leading tothe development of IgE-mediated food allergy.

Item Type: Article
Authors/Creators:Lee, KH and Guo, J and Song, Y and Ariff, A and O'Sullivan, M and Hales, B and Mullins, BJ and Zhang, G
Keywords: 16S rRNA gene sequencing, food allergy, microbiome, WGCNA, Ruminococcaceae
Journal or Publication Title: International Journal of Molecular Sciences
Publisher: Molecular Diversity Preservation International
ISSN: 1422-0067
DOI / ID Number: 10.3390/ijms22042079
Copyright Information:

Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/).

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