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Lack of N-terminal segment of the flagellin protein results in the production of a shortened polar flagellum in the deep-sea sedimentary bacterium Pseudoalteromonas sp. strain SM9913

Sheng, Q, Liu, S-M, Cheng, J-H, Li, C-Y, Fu, H-H, Zhang, X-Y, Song, X-Y, McMinn, A ORCID: 0000-0002-2133-3854, Zhang, Y-Z, Su, H-N and Chen, X-L 2021 , 'Lack of N-terminal segment of the flagellin protein results in the production of a shortened polar flagellum in the deep-sea sedimentary bacterium Pseudoalteromonas sp. strain SM9913' , Applied and Environmental Microbiology, vol. 87, no. 21 , pp. 1-14 , doi: 10.1128/AEM.01527-21.

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Abstract

Bacterial polar flagella, comprised of flagellin, are essential for bacterial motility. Pseudoalteromonas sp. strain SM9913 is a bacterium isolated from deep-sea sediments. Unlike other Pseudoalteromonas strains that have a long polar flagellum, strain SM9913 has an abnormally short polar flagellum. Here, we investigated the underlying reason for the short flagellum and found that a single-base mutation was responsible for the altered flagellar assembly. This mutation leads to the fragmentation of the flagellin gene into two genes, PSM_A2281, encoding the core segment and the C-terminal segment, and PSM_A2282, encoding the N-terminal segment, and only gene PSM_A2281 is involved in the production of the short polar flagellum. When a chimeric gene of PSM_A2281 and PSM_A2282 encoding an intact flagellin, A2281::82, was expressed, a long polar flagellum was produced, indicating that the N-terminal segment of flagellin contributes to the production of a polar flagellum of a normal length. Analyses of the simulated structures of A2281 and A2281::82 and that of the flagellar filament assembled with A2281::82 indicate that due to the lack of two α-helices, the core of the flagellar filament assembled with A2281 is incomplete and is likely too weak to support the stability and movement of a long flagellum. This mutation in strain SM9913 had little effect on its growth and only a small effect on its swimming motility, implying that strain SM9913 can live well with this mutation in natural sedimentary environments. This study provides a better understanding of the assembly and production of bacterial flagella.

Item Type: Article
Authors/Creators:Sheng, Q and Liu, S-M and Cheng, J-H and Li, C-Y and Fu, H-H and Zhang, X-Y and Song, X-Y and McMinn, A and Zhang, Y-Z and Su, H-N and Chen, X-L
Keywords: Pseudoalteromonas, flagellum, deep sea, flagellin, N-terminal segment, ND0 and ND1 domains, flagellar assembly, flagellar length, bacterial motility, swimming, flagellin length
Journal or Publication Title: Applied and Environmental Microbiology
Publisher: Amer Soc Microbiology
ISSN: 0099-2240
DOI / ID Number: 10.1128/AEM.01527-21
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Copyright © 2021 American Society for Microbiology. All Rights Reserved.

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