Open Access Repository

High-resolution mapping of the Alp locus and identification of a candidate gene HvMate controlling aluminium tolerance in barley (Hordeum vulgare L.)

Downloads

Downloads per month over past year

Wang, J and Raman, H and Zhou, M and Ryan, PR and Delhaize, E and Hebb, DM and Coombes, N and Mendham, NJ (2007) High-resolution mapping of the Alp locus and identification of a candidate gene HvMate controlling aluminium tolerance in barley (Hordeum vulgare L.). Theoretical and Applied Genetics, 115 (2). pp. 265-276. ISSN 0040-5752

[img] PDF
3897.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

Aluminium (Al) tolerance in barley is conditioned
by the Alp locus on the long arm of chromosome 4H,
which is associated with Al-activated release of citrate from roots. We developed a high-resolution map of the Alp locus using 132 doubled haploid (DH) lines from a cross between Dayton (Al-tolerant) and Zhepi 2 (Al-sensitive) and 2,070 F2 individuals from a cross between Dayton and Gairdner (Al-sensitive). The Al-activated eZux of citrate from the root apices of Al-tolerant Dayton was 10-fold greater than from the Al-sensitive parents Zhepi 2 and Gairdner. A suite of markers (ABG715, Bmag353, GBM1071, GWM165, HvMATE and HvGABP) exhibited complete linkage with
the Alp locus in the DH population accounting 72% of the
variation for Al tolerance evaluated as relative root elongation. These markers were used to map this genomic region in the Dayton/Gairdner population in more detail. Flanking markers HvGABP and ABG715 delineated the Alp locus to a 0.2 cM interval. Since the HvMATE marker was not
polymorphic in the Dayton/Gairdner population we instead
investigated the expression of the HvMATE gene. Relative
expression of the HvMATE gene was 30-fold greater in
Dayton than Gardiner. Furthermore, HvMATE expression
in the F2:3 families tested, including all the informative
recombinant lines identified between HvGABP and
ABG715 was significantly correlated with Al tolerance and
Al-activated citrate eZux. These results identify HvMATE,
a gene encoding a multidrug and toxic compound extrusion
protein, as a candidate controlling Al tolerance in barley.

Item Type: Article
Keywords: Aluminium tolerance · Barley · Citrate eZux · Comparative mapping · MATE · Molecular markers
Journal or Publication Title: Theoretical and Applied Genetics
Publisher: Springer-Verlag
Page Range: pp. 265-276
ISSN: 0040-5752
Identification Number - DOI: 10.1007/s00122-007-0562-9
Additional Information:

The original publication is available at www.springerlink.com

Date Deposited: 07 Apr 2008 14:11
Last Modified: 18 Nov 2014 03:33
Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page
TOP