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High-resolution mapping of the Alp locus and identification of a candidate gene HvMate controlling aluminium tolerance in barley (Hordeum vulgare L.)


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Wang, J, Raman, H, Zhou, M, Ryan, PR, Delhaize, E, Hebb, DM, Coombes, N and Mendham, NJ 2007 , 'High-resolution mapping of the Alp locus and identification of a candidate gene HvMate controlling aluminium tolerance in barley (Hordeum vulgare L.)' , Theoretical and Applied Genetics, vol. 115, no. 2 , pp. 265-276 , doi: 10.1007/s00122-007-0562-9.

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Aluminium (Al) tolerance in barley is conditioned
by the Alp locus on the long arm of chromosome 4H,
which is associated with Al-activated release of citrate from roots. We developed a high-resolution map of the Alp locus using 132 doubled haploid (DH) lines from a cross between Dayton (Al-tolerant) and Zhepi 2 (Al-sensitive) and 2,070 F2 individuals from a cross between Dayton and Gairdner (Al-sensitive). The Al-activated eZux of citrate from the root apices of Al-tolerant Dayton was 10-fold greater than from the Al-sensitive parents Zhepi 2 and Gairdner. A suite of markers (ABG715, Bmag353, GBM1071, GWM165, HvMATE and HvGABP) exhibited complete linkage with
the Alp locus in the DH population accounting 72% of the
variation for Al tolerance evaluated as relative root elongation. These markers were used to map this genomic region in the Dayton/Gairdner population in more detail. Flanking markers HvGABP and ABG715 delineated the Alp locus to a 0.2 cM interval. Since the HvMATE marker was not
polymorphic in the Dayton/Gairdner population we instead
investigated the expression of the HvMATE gene. Relative
expression of the HvMATE gene was 30-fold greater in
Dayton than Gardiner. Furthermore, HvMATE expression
in the F2:3 families tested, including all the informative
recombinant lines identified between HvGABP and
ABG715 was significantly correlated with Al tolerance and
Al-activated citrate eZux. These results identify HvMATE,
a gene encoding a multidrug and toxic compound extrusion
protein, as a candidate controlling Al tolerance in barley.

Item Type: Article
Authors/Creators:Wang, J and Raman, H and Zhou, M and Ryan, PR and Delhaize, E and Hebb, DM and Coombes, N and Mendham, NJ
Keywords: Aluminium tolerance · Barley · Citrate eZux · Comparative mapping · MATE · Molecular markers
Journal or Publication Title: Theoretical and Applied Genetics
Publisher: Springer-Verlag
ISSN: 0040-5752
DOI / ID Number: 10.1007/s00122-007-0562-9
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