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Effect of cloned inhibitor resistant TEM B-lactamases on the susceptibility of H. influenzae to amoxicillin/clavulanate

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Tristram, SG and Burdach, JG (2007) Effect of cloned inhibitor resistant TEM B-lactamases on the susceptibility of H. influenzae to amoxicillin/clavulanate. Journal of Antimicrobial Chemotherapy, 60 (5). pp. 1151-1154. ISSN 0305-7453

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Abstract

Objectives: To determine the effect of cloned inhibitor-resistant TEM b-lactamases (IRTs) on the
susceptibility of Haemophilus influenzae to amoxicillin/clavulanate.
Methods: IRT-2, -4 and -5 genes with various promoters were cloned into control strains of H. influenzae
and the amoxicillin/clavulanate MICs were measured using Etests.
Results: IRT enzymes were able to raise the amoxicillin/clavulanate MICs to between 0.38/0.19 and 4.0/
2.0 mg/L depending on the IRT and promoter genotype, compared with MICs of 0.19/0.09 to 0.5/
0.25 mg/L for the corresponding strains with TEM-1. Strains with an IRT and altered penicillin-binding
proteins had amoxicillin/clavulanate MICs as high as 8.0/4.0 mg/L.
Conclusions: Cloned IRT enzymes in H. influenzae raise the amoxicillin/clavulanate MICs to an extent
comparable to naturally occurring strains with decreased amoxicillin/clavulanate susceptibility.

Item Type: Article
Keywords: H. influenzae, IRTs, susceptibility
Journal or Publication Title: Journal of Antimicrobial Chemotherapy
Publisher: Oxford University Press
Page Range: pp. 1151-1154
ISSN: 0305-7453
Identification Number - DOI: 10.1093/jac/dkm311
Additional Information:

The definitive publisher-authenticated version http://www.oxfordjournals.org/

Date Deposited: 07 Apr 2008 14:12
Last Modified: 18 Nov 2014 03:33
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