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Effect of cloned inhibitor resistant TEM B-lactamases on the susceptibility of H. influenzae to amoxicillin/clavulanate


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Tristram, SG and Burdach, JG 2007 , 'Effect of cloned inhibitor resistant TEM B-lactamases on the susceptibility of H. influenzae to amoxicillin/clavulanate' , Journal of Antimicrobial Chemotherapy, vol. 60, no. 5 , pp. 1151-1154 , doi: 10.1093/jac/dkm311.

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Objectives: To determine the effect of cloned inhibitor-resistant TEM b-lactamases (IRTs) on the
susceptibility of Haemophilus influenzae to amoxicillin/clavulanate.
Methods: IRT-2, -4 and -5 genes with various promoters were cloned into control strains of H. influenzae
and the amoxicillin/clavulanate MICs were measured using Etests.
Results: IRT enzymes were able to raise the amoxicillin/clavulanate MICs to between 0.38/0.19 and 4.0/
2.0 mg/L depending on the IRT and promoter genotype, compared with MICs of 0.19/0.09 to 0.5/
0.25 mg/L for the corresponding strains with TEM-1. Strains with an IRT and altered penicillin-binding
proteins had amoxicillin/clavulanate MICs as high as 8.0/4.0 mg/L.
Conclusions: Cloned IRT enzymes in H. influenzae raise the amoxicillin/clavulanate MICs to an extent
comparable to naturally occurring strains with decreased amoxicillin/clavulanate susceptibility.

Item Type: Article
Authors/Creators:Tristram, SG and Burdach, JG
Keywords: H. influenzae, IRTs, susceptibility
Journal or Publication Title: Journal of Antimicrobial Chemotherapy
Publisher: Oxford University Press
ISSN: 0305-7453
DOI / ID Number: 10.1093/jac/dkm311
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