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Ultrastructural examination of the host cellular response in the gills of Atlantic salmon, Salmo salar, with amoebic gill disease

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Lovy, J and Becker, J and Speare, J and Wadowski, DW and Wright, GM and Powell, MD (2007) Ultrastructural examination of the host cellular response in the gills of Atlantic salmon, Salmo salar, with amoebic gill disease. Veterinary Pathology, 44 (5). pp. 663-671. ISSN 0300-9858

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Abstract

Gills from Atlantic salmon with experimentally induced amoebic gill disease ( Neoparamoeba spp.) were examined with transmission electron microscopy to assess pathology and host-cell responses. Amoebae were found either on the surface epithelium or with pseudopodia extending deeply into invaginations of epithelial cells. The amoebae had various densities along the plasma membrane and contained electron-dense deposits within their cytoplasm. Surface epithelial cells sloughed from the gills and had features consistent with apoptosis, including rounded shape, loss of surface microridges, and hypercondensation of nuclear chromatin. Affected areas of gills had fusion of secondary lamellae with interlamellar spaces occupied by mitotic epithelial cells and eosinophils. Eosinophils contained abundant fusiform-shaped granules that measured approximately 1 µm long and 360 nm wide. The granule consisted of an electron-dense matrix with a central inclusion that was less electron-dense, consisting of particulate and fibrillar material. In many instances, the central inclusion appeared empty and 90% of the eosinophils had morphology suggestive of piecemeal degranulation. Also observed within affected areas were a few neutrophils, mucous cells releasing mucus, and a small number of dendritic-like cells.

Item Type: Article
Keywords: Amoebic gill disease; electron microscopy; eosinophils; fish; gills.
Journal or Publication Title: Veterinary Pathology
Publisher: American College of Veterinary Pathologists
Page Range: pp. 663-671
ISSN: 0300-9858
Additional Information:

© 2007 American College of Veterinary Pathologists

Date Deposited: 07 Apr 2008 14:13
Last Modified: 18 Nov 2014 03:33
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