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Effect of olfactory ensheathing cells on reactive astrocytes in vitro


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O'Toole, DAJ, West, AK and Chuah, MI 2007 , 'Effect of olfactory ensheathing cells on reactive astrocytes in vitro' , Cellular and Molecular Life Sciences, vol. 64, no. 10 , pp. 1303-1309 , doi: 10.1007/s00018-007-7106-y.

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Olfactory ensheathing cells have been used
in several studies to promote repair in the injured
spinal cord. However, cellular interaction between
olfactory ensheathing cells and glial cells induced to
be reactive in the aftermath of injury site has not been
investigated. Using an in vitro model of astrogliosis,
we show that reactive astrocytes expressed significantly
less glial fibrillary acidic protein (GFAP) when
cultured both in direct contact with olfactory ensheathing
cells and when the two cell types were
separated by a porous membrane. Immunofluorescence
staining also suggested that reactive astrocytes
showed decreased chondroitin sulfate proteoglycans
in the presence of olfactory ensheathing cells, although
the reduction was not statistically significant.
No down-regulation of GFAP was observed when
reactive astrocytes were similarly cultured with
Schwann cells. Cell viability assay and bromodeoxyuridine
uptake showed that proliferation of reactive
astrocytes was significantly increased in the presence
of olfactory ensheathing cells and Schwann cells.

Item Type: Article
Authors/Creators:O'Toole, DAJ and West, AK and Chuah, MI
Keywords: Olfactory ensheathing cells, reactive astrocytes, glial fibrillary acidic protein, proliferation, astrogliosis.
Journal or Publication Title: Cellular and Molecular Life Sciences
Publisher: Birkhauser Verlag
ISSN: 1420-682X
DOI / ID Number: 10.1007/s00018-007-7106-y
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