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Abstract

Preparation of monolithic capillary columns for separations in the CEC mode using
UV-initiated polymerization of the plain monolith followed by functionalization of
its pore surface by photografting has been studied. The first step enabled the preparation
of generic poly(butyl methacrylate-co-ethylene dimethacrylate) monoliths
with optimized porous properties, controlled by the percentages of porogens 1-
decanol and cyclohexanol in the polymerization mixture, irradiation time, and UV
light intensity. Ionizable monomers [2-(methacryloyloxy)ethyl]trimethylammonium
chloride or 2-acryloamido-2-methyl-1-propanesulfonic acid were then photografted
onto the monolithic matrix, allowing us to control the direction of the EOF in CEC.
Different strategies were applied to control the grafting density and, thereby, the
magnitude of the EOF. To control the hydrophobic properties, two approaches were
tested: (i) cografting of a mixture of the ionizable and hydrophobic monomers and
(ii) sequential grafting of the ionizable and hydrophobic monomers. Cografting
resulted in similar retention but higher EOF. With sequential grafting, more than
50% increase in retention factors was obtained and a slight decrease in EOF was
observed due to shielding of the ionizable moieties.

Item Type:	Article
Authors/Creators:	Eeltink, S and Hilder, EF and Geiser, L and Svec, F and Frechet, JMJ and Rozing, GP and Schoenmakers, PJ and Kok, WT
Keywords:	Capillary electrochromatography / Monolithic columns / Photografting / Selectivity
Journal or Publication Title:	Journal of Separation Science
Publisher:	Wiley-VCH
ISSN:	1615-9306
DOI / ID Number:	10.1002/jssc.200600316
Additional Information:	The original publication is available at http://www.interscience.wiley.com/
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