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Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR


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Park, TG, Bell, EM, Pearce, I, Rublee, PA, Bolch, CJS and Hallegraeff, GM 2007 , 'Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR' , Polar Biology, vol. 30, no. 4 , pp. 843-848 , doi: 10.1007/s00300-006-0244-0.

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The heterotrophic dinoXagellate PWesteria
piscicida was detected in Ace Lake in the Vestfold
Hills, eastern Antarctica by using real-time PCR based
on 18S rDNA sequences. Antarctic water samples collected
in 2004 were tested by species-speciWc real-time
PCR assays for the identiWcation of P. piscicida and
P. shumwayae. Positive results were shown with
P. piscicida-speciWc real-time PCR, and PCR products
were examined by sequence analysis for conWrmation.
A phylogenetic tree made from partial 18S rDNA
sequences showed that the Antarctic clone clustered
with P. piscicida. This result suggests that P. piscicida is
present in the extreme conditions of an Antarctic
saline lake which has not contained Wsh for thousands
of years.

Item Type: Article
Authors/Creators:Park, TG and Bell, EM and Pearce, I and Rublee, PA and Bolch, CJS and Hallegraeff, GM
Journal or Publication Title: Polar Biology
Publisher: Springer-Verlag
ISSN: 0722-4060
DOI / ID Number: 10.1007/s00300-006-0244-0
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