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Proteomics identifies enhanced expression of stefin A in neonatal murine skin compared with adults: functional implications


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Scott, DK, Lord, R, Muller, HK, Malley, RC and Woods, GM 2007 , 'Proteomics identifies enhanced expression of stefin A in neonatal murine skin compared with adults: functional implications' , British Journal of Dermatology, vol. 156, no. 6 , pp. 1156-1162 , doi: 10.1111/j.1365-2133.2007.07875.x.

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Background Skin develops through a process of epidermal proliferation, maturation,
and remodelling of the epidermis and dermis. This period also involves the maturation
of the skin immune system, such that antigen applied though the skin
of a neonatal mouse always results in immunosuppression, whereas in adults,
immunity will occur.
Objectives Using proteomics, to identify proteins uniquely involved in the development
of the skin and skin immune system.
Methods Proteins were extracted from whole skin of mice aged 4 and 21 days, and
separated using two-dimensional electrophoresis.
Results Of the 25 proteins that were sequenced by peptide mass fingerprinting
with matrix-assisted laser desorption/ionization–time of flight–mass spectrometry,
three were known markers of keratinocyte differentiation and proliferation.
These were cyclophilin A, epidermal fatty acid binding protein 5 and
stefin A. Of interest were the two isoforms of stefin A, an intracellular protease
inhibitor, found in neonatal skin. The strong expression of stefin A in neonates
was confirmed by immunohistochemical analysis, suggesting an important role
in the development of the epidermis. Additionally, Western blotting identified
two larger isoforms in adult skin, revealing a change in the stefin A during
Conclusions We propose that stefin A is involved in development of the skin, that
development of the skin and of immune function is linked, and that stefin A has
an important function in neonatal skin and potentially the neonatal immune

Item Type: Article
Authors/Creators:Scott, DK and Lord, R and Muller, HK and Malley, RC and Woods, GM
Keywords: cystatin A, neonatal tolerance, proteomics, skin immune system, stefin A, two-dimensional electrophoresis
Journal or Publication Title: British Journal of Dermatology
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 0385-2407
DOI / ID Number: 10.1111/j.1365-2133.2007.07875.x
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