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Evaluation of quantitative polymerase chain reaction to assess nosZ gene prevalence in mixed microbial communities

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Sicilliano, SD and Wai, M and Powell, S (2007) Evaluation of quantitative polymerase chain reaction to assess nosZ gene prevalence in mixed microbial communities. Canadian Journal of Microbiology, 53 (5). pp. 636-642. ISSN 0008-4166

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Abstract

The usefulness of quantitative polymerase chain reaction (QPCR) to measure nosZ gene prevalence in a multi-template reaction was assessed by comparing 19 nosZ template DNA samples and 91 model communities. Efficiencies of the QPCR varied but were not significantly different among nosZ genotypes and were not linked to genetic distance from Ralstonia eutropha. nosZ genotype QPCR efficiencies obtained from isolated denitrifiers were higher (84.8%) than those obtained from excised denaturing gradient gel electrophoresis bands or clones of PCR products from total community DNA (ca. 60%). Analysis of the model communities indicated that QPCR accurately predicts gene prevalence in communities composed of up to six templates.

Item Type: Article
Keywords: denitrification, nosZ, quantitative PCR, microbial ecology, Antarctic, Arctic, soils.
Journal or Publication Title: Canadian Journal of Microbiology
Publisher: NRC Research Press
Page Range: pp. 636-642
ISSN: 0008-4166
Identification Number - DOI: 10.1139/W07-014
Additional Information:

© 2007 NRC Canada. Please refer to the journal link for access to the definitive, published version. Under the Canadian Copyright Act, individuals may download or print single copies of articles for personal research or study. Any person may reproduce short excerpts from articles in the journals for any purpose that respects the moral rights of authors, provided that the source is fully acknowledged. As a courtesy, the consent of authors of such material should be obtained directly from the author.

Date Deposited: 07 Apr 2008 14:22
Last Modified: 18 Nov 2014 03:34
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