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A randomized controlled clinical trial to determine the optimum duration of G-CSF priming prior to BM stem cell harvesting

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Lowenthal, RM and Ragg, SJ and Anderson, J and Nicholson, L and Harrup, R and Tuck, DM (2007) A randomized controlled clinical trial to determine the optimum duration of G-CSF priming prior to BM stem cell harvesting. Cytotherapy, 9 (2). pp. 158-164. ISSN 1465-3249

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Abstract

Background.
Harvesting of hemopoietic stem cells (HSC) from G-CSF-primed BM for autologous transplantation is an alternative to collection of unprimed BM or G-CSF-primed peripheral blood (PB). However, the optimum number of days of G-CSF administration for this purpose is unknown. We set out to determine whether cell yields could be optimized by varying the number of days of G-CSF administration
prior to BM stem cell harvesting.

Methods.
We conducted a randomized controlled single-center trial of 6 days(the standard) vs. 4 days of G-CSF administration and compared yields of total nucleated cells (TNC), CD34� HSC and CFU-GM cells per kilogram patient body weight. Statistical analysis was by Student’s t-test.

Results.
Twenty-four patients were enrolled; 13 received 6 days and 11 received 4 days of G-CSF administration. Analysis of the first harvest aspirate showed higher proportions of CD34� HSC (P�/0.02) and CFU-GM (P�/0.03) in the 4-day group. For the 6-day and 4-day groups, respectively, the median yield of TNC/kg was 6.5�/108 and 5.4�/108 (P�/0.28), of CD34� cells/kg 0.56�/106 and 0.98�/106
(P�/0.04) and of CFU-GM cells/kg 1.66�/105 and 1.55�/105
(P�/0.75).

Discussion.
These results suggest that by 6 days the HSC-stimulating effect of GCSF has passed its peak and that 4 days should be adopted as the standard for G-CSF priming prior to BM stem cell harvesting for autologous transplantation.

Item Type: Article
Keywords: autologous, BMT, G-CSF, harvesting, yields.
Journal or Publication Title: Cytotherapy
Publisher: TAYLOR & FRANCIS AS,
Page Range: pp. 158-164
ISSN: 1465-3249
Identification Number - DOI: 10.1080/14653240601182820
Additional Information:

The definitive version is available online at
http://www.informaworld.com/smpp/content?

Copyright © 2007 Taylor & Francis

Date Deposited: 07 Apr 2008 14:22
Last Modified: 18 Nov 2014 03:34
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