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Methanobrevibacter millerae sp. nov. and Methanobrevibacter olleyae sp. nov., methanogens from the ovine and bovine rumen that can utilize formate for growth

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Rea, S and Bowman, JP and Popovski, S and Pimm, C and Wright, AG (2007) Methanobrevibacter millerae sp. nov. and Methanobrevibacter olleyae sp. nov., methanogens from the ovine and bovine rumen that can utilize formate for growth. International Journal of Systematic and Evolutionary, 57. pp. 450-456. ISSN 1466-5026

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Abstract

Four formate-utilizing methanogens were isolated from ovine (strain KM1H5-1PT) and bovine
(strains AK-87, OCP and ZA-10T) rumen contents. Based on 16S rRNA gene sequence analysis,
the methanogen strains were found to belong to the order Methanobacteriales in the genus
Methanobrevibacter. Strains ZA-10T and KM1H5-1PT gained energy for growth by the reduction of
CO2 to CH4 using H2 or formate exclusively as electron donors. Increasing formate concentrations
to 220 mM in batch cultures increased the growth of strain KM1H5-1PT but did not affect the
growth of strain ZA-10T. Substrate specificity and resistance to cell-wall lysis supported the
affiliation of the strains to the genus Methanobrevibacter. Strains ZA-10T and KM1H5-1PT showed
16S rRNA gene sequence similarity of 98.0 and 98.6% to their closest recognized relatives,
Methanobrevibacter thaueri CWT and Methanobrevibacter ruminantium M1T, respectively.
DNA–DNA hybridization experiments indicated that the strains were not affiliated at the species
level to their closest recognized relatives, with DNA reassociation values of only 28% between
strains ZA-10T and Methanobrevibacter thaueri CWT and <25% between strains KM1H5-1PT
and Methanobrevibacter ruminantium M1T. Based on the data presented, the new strains are
considered to represent two novel species of the genus Methanobrevibacter, for which the names
Methanobrevibacter millerae sp. nov. (type strain ZA-10T=DSM 16643T=OCM 820T) and
Methanobrevibacter olleyae sp. nov. (type strain KM1H5-1PT=DSM 16632T=OCM 841T) are
proposed.

Item Type: Article
Journal or Publication Title: International Journal of Systematic and Evolutionary
Publisher: Society for General Microbiology
Page Range: pp. 450-456
ISSN: 1466-5026
Identification Number - DOI: 10.1099/ijs.0.63984-0
Date Deposited: 07 Apr 2008 14:27
Last Modified: 18 Nov 2014 03:34
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