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Identification and expression of natriuretic peptide receptor type-A and -B mRNA in freshwater and seawater rainbow trout

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Nankervis, S and Powell, MD and McLeod, J and Toop, T (2007) Identification and expression of natriuretic peptide receptor type-A and -B mRNA in freshwater and seawater rainbow trout. Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology, 177 (2). pp. 259-267. ISSN 1096-4959

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Abstract

Natriuretic peptide receptors mediate the
physiological response of natriuretic peptide hormones.
One of the natriuretic peptide receptor types is
the particulate guanylyl cyclase receptors, of which
there are two identiWed: NPR-A and NPR-B. In Wshes,
these have been sequenced and characterized in eels,
medaka, and dogWsh shark (NPR-B only). The euryhaline
rainbow trout provides an opportunity to further
pursue examination of the system in teleosts. In this
study, partial rainbow trout NPR-A-like and NPR-Blike
mRNA sequences were identiWed via PCR and
cloning. The sequence information was used in realtime
PCR to examine mRNA expression in a variety of
tissues of freshwater rainbow trout and rainbow trout
acclimated to 35 parts per thousand seawater for a
period of 10 days. In the excretory kidney and posterior
intestine, real-time PCR analysis showed greater
expression of NPR-B in freshwater Wsh than in those
adapted to seawater; otherwise, there was no diVerence
in the expression of the individual receptors in fresh
water or seawater. In general, the expression of the
NPR-A and NPR-B type receptors was quite low.

Item Type: Article
Keywords: Natriuretic peptides - Fish osmoregulation - Rainbow trout - mRNA expression - Natriuretic peptide receptors
Journal or Publication Title: Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology
Publisher: Springer Netherlands
Page Range: pp. 259-267
ISSN: 1096-4959
Identification Number - DOI: 10.1007/s00360-006-0127-2
Additional Information:

The original publication is available at www.springerlink.com

Date Deposited: 07 Apr 2008 14:27
Last Modified: 18 Nov 2014 03:34
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