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Neoparamoeba perurans n. sp., an agent of amoebic gill disease of Atlantic Salmon (Salmo salar)

Young, ND, Crosbie, PBB, Adams, MB, Nowak, BF and Morrison, RN 2007 , 'Neoparamoeba perurans n. sp., an agent of amoebic gill disease of Atlantic Salmon (Salmo salar)' , International Journal for Parasitology, vol. 37, no. 13 , pp. 1469-1481 , doi: 10.1016/j.ijpara.2007.04.018.

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Amoebic gill disease (AGD) is a potentially fatal disease of some marine fish. Two amphizoic amoebae Neoparamoeba pemaquidensis and Neoparamoeba branchiphila have been cultured from AGD-affected fish, yet it is not known if one or both are aetiological agents. Here, we PCR amplified the 18S rRNA gene of non-cultured, gill-derived (NCGD) amoebae from AGD-affected Atlantic salmon (Salmo salar) using N. pemaquidensis and N. branchiphila-specific oligonucleotides. Variability in PCR amplification led to comparisons of 18S rRNA and 28S rRNA gene sequences from NCGD and clonal cultured, gill-derived (CCGD) N. pemaquidensis and N. branchiphila. Phylogenetic analyses inferred from either 18S or 28S rRNA gene sequences unambiguously segregated a lineage consisting of NCGD amoebae from other members of the genus Neoparamoeba. Species-specific oligonucleotide probes that hybridise 18S rRNA were designed, validated and used to probe gill tissue from AGD-affected Atlantic salmon. The NCGD amoebae-specific probe bound AGD-associated amoebae while neither N. pemaquidensis nor N. branchiphila were associated with AGD-lesions. Together, these data indicate that NCGD amoebae are a new species, designated Neoparamoeba perurans n.sp. and this is the predominant aetiological agent of AGD of Atlantic salmon cultured in Tasmania, Australia.

Item Type: Article
Authors/Creators:Young, ND and Crosbie, PBB and Adams, MB and Nowak, BF and Morrison, RN
Keywords: 18S; 28S; Amoebic gill disease; In situ hybridisation; Neoparamoeba branchiphila; Neoparamoeba pemaquidensis; Neoparamoeba perurans; rRNA; Salmo salar
Journal or Publication Title: International Journal for Parasitology
Publisher: Elsevier Ltd
ISSN: 0020-7519
DOI / ID Number: 10.1016/j.ijpara.2007.04.018
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