Please Note:

The Open Access Repository has moved to a new authentication system as of the 1st of November.

Account holders will now be able to login using their University of Tasmania credentials.
If you have trouble logging in please email us on so we can assist you.

Public users can still access the records in this repository as normal

Open Access Repository

Methyl-ß-cyclodextrin reversibly alters the gating of lipid rafts-associated Kv1.3 channels in Jurkat T lymphocytes


Downloads per month over past year

Pottosin, II and Valencia-Cruz, G and Bonales-Alatorre, E and Shabala, SN and Dobrovinskaya, OR (2007) Methyl-ß-cyclodextrin reversibly alters the gating of lipid rafts-associated Kv1.3 channels in Jurkat T lymphocytes. Pfluegers Archiv: European journal of physiology, 454 (2). pp. 235-244. ISSN 0031-6768

[img] PDF
4309.pdf | Request a copy
Full text restricted


The voltage-dependent Kv1.3 potassium channels
mediate a variety of physiological functions in human
T lymphocytes. These channels, along with their multiple
regulatory components, are localized in cholesterolenriched
microdomains of plasma membrane (lipid rafts).
In this study, patch-clamp technique was applied to explore
the impact of the lipid-raft integrity on the Kv1.3 channel
functional characteristics. T lymphoma Jurkat cells were
treated for 1 h with cholesterol-binding oligosaccharide
methyl-β-cyclodextrin (MβCD) in 1 or 2 mM concentration,
resulting in depletion of cholesterol by 63±5 or 75±
4%, respectively. Treatment with 2 mM MβCD did not
affect the cells viability but retarded the cell proliferation.
The latter treatment caused a depolarizing shift of the Kv1.3
channel activation and inactivation by 11 and 6 mV,
respectively, and more than twofold decrease in the
steady-state activity at depolarizing potentials. Altogether,
these changes underlie the depolarization of membrane
potential, recorded in a current-clamp mode. The effects of
MβCD were concentration- and time-dependent and reversible.
Both development and recovery of the MβCD
effects were completed within 1–2 h. Therefore, cholesterol
depletion causes significant alterations in the Kv1.3 channel
function, whereas T cells possess a potential to reverse
these changes.

Item Type: Article
Journal or Publication Title: Pfluegers Archiv: European journal of physiology
Publisher: Springer
Page Range: pp. 235-244
ISSN: 0031-6768
Identification Number - DOI: 10.1007/s00424-007-0208-4
Additional Information:

The original publication is available at

Date Deposited: 07 Apr 2008 14:32
Last Modified: 18 Nov 2014 03:35
Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page