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Methyl-ß-cyclodextrin reversibly alters the gating of lipid rafts-associated Kv1.3 channels in Jurkat T lymphocytes


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Pottosin, II, Valencia-Cruz, G, Bonales-Alatorre, E, Shabala, SN and Dobrovinskaya, OR 2007 , 'Methyl-ß-cyclodextrin reversibly alters the gating of lipid rafts-associated Kv1.3 channels in Jurkat T lymphocytes' , Pfluegers Archiv: European journal of physiology, vol. 454, no. 2 , pp. 235-244 , doi: 10.1007/s00424-007-0208-4.

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The voltage-dependent Kv1.3 potassium channels
mediate a variety of physiological functions in human
T lymphocytes. These channels, along with their multiple
regulatory components, are localized in cholesterolenriched
microdomains of plasma membrane (lipid rafts).
In this study, patch-clamp technique was applied to explore
the impact of the lipid-raft integrity on the Kv1.3 channel
functional characteristics. T lymphoma Jurkat cells were
treated for 1 h with cholesterol-binding oligosaccharide
methyl-β-cyclodextrin (MβCD) in 1 or 2 mM concentration,
resulting in depletion of cholesterol by 63±5 or 75±
4%, respectively. Treatment with 2 mM MβCD did not
affect the cells viability but retarded the cell proliferation.
The latter treatment caused a depolarizing shift of the Kv1.3
channel activation and inactivation by 11 and 6 mV,
respectively, and more than twofold decrease in the
steady-state activity at depolarizing potentials. Altogether,
these changes underlie the depolarization of membrane
potential, recorded in a current-clamp mode. The effects of
MβCD were concentration- and time-dependent and reversible.
Both development and recovery of the MβCD
effects were completed within 1–2 h. Therefore, cholesterol
depletion causes significant alterations in the Kv1.3 channel
function, whereas T cells possess a potential to reverse
these changes.

Item Type: Article
Authors/Creators:Pottosin, II and Valencia-Cruz, G and Bonales-Alatorre, E and Shabala, SN and Dobrovinskaya, OR
Journal or Publication Title: Pfluegers Archiv: European journal of physiology
Publisher: Springer
ISSN: 0031-6768
DOI / ID Number: 10.1007/s00424-007-0208-4
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