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Insulin and contractions increase nutritive blood flow in rat muscle in vivo: determined by microdialysis of L-[14C]glucose


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Newman, JMB and Ross, RM and Richards, SM and Clark, MG and Rattigan, S (2007) Insulin and contractions increase nutritive blood flow in rat muscle in vivo: determined by microdialysis of L-[14C]glucose. Journal of Physiology, 585 (1). pp. 217-229. ISSN 0022-3751

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In the present study, a mathematical model using the microdialysis outflow : inflow (O/I) ratio
of the novel analogue L-[14C]glucose has been developed which allows the calculation of the
nutritive (and non-nutritive) flow in muscle as a proportion of total blood flow. Anaesthetized
rats had microdialysis probes carrying L-[14C]glucose inserted through a calf muscle group
(tibialis/plantaris/gastrocnemius). The nutritive fraction of total blood flow was determined
under basal conditions and in response to contraction (electrical field stimulation), insulin
(hyperinsulinaemic euglycaemic clamp with 10mUmin−1 kg−1 insulin) or saline control from
limb blood flow and the microdialysis O/I ratio of L-[14C]glucose. Both contraction and
insulin infusion decreased the O/I ratio of L-[14C]glucose and increased total limb blood
flow. Calculations based on mathematical models using L-[14C]glucose O/I and limb blood
flow revealed that during basal conditions, the nutritive fraction of total flow was 0.38±0.06,
indicating that basal flow was predominantly non-nutritive. Contraction and insulin increased
the nutritive fraction to 0.82±0.24 (P<0.05) and 0.52±0.12 (P<0.05). Thus the increase in
limb blood flow from insulin was fully accommodated by nutritive flow, while contraction
increased nutritive flow at the expense of non-nutritive flow. This novel method using
microdialysis and the O/I ratio of L-[14C]glucose allows the determination of the nutritive fraction
of total flow in muscle as well as the proportion of total flow that may be redistributed in response
to contraction and insulin.

Item Type: Article
Journal or Publication Title: Journal of Physiology
Publisher: Blackwell Publishing
Page Range: pp. 217-229
ISSN: 0022-3751
Identification Number - DOI: 10.1113/jphysiol.2007.138818
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Date Deposited: 07 Apr 2008 14:34
Last Modified: 18 Nov 2014 03:35
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