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Bacteria and PAMPs activate NK?B and Gro production in a subset of olfactory ensheathing cells and astrocytes but not in Schwann cells

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Vincent, AJ and Choi-Lundberg, DL and Harris, JA and West, AK and Chuah, MI (2007) Bacteria and PAMPs activate NK?B and Gro production in a subset of olfactory ensheathing cells and astrocytes but not in Schwann cells. Glia, 55 (9). pp. 905-916. ISSN 0894-1491

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Abstract

The primary olfactory nerves provide uninterrupted conduits
for neurotropic pathogens to access the brain from the
nasal cavity, yet infection via this route is uncommon. It is
conceivable that olfactory ensheathing cells (OECs), which
envelope the olfactory nerves along their entire length, provide
a degree of immunological protection against such
infections. We hypothesized that cultured OECs would be
able to mount a biologically significant response to bacteria
and pathogen-associated molecular patterns (PAMPs). The
response of OECs to Escherichia coli (E. coli) and various
PAMPs was compared to that of Schwann cells (SCs), astrocytes
(ACs), and microglia (MG). A subset of OECs displayed
nuclear localization of nuclear factor kB (NFkB), an
inflammatory transcription factor, after treatment with E.
coli (20% 6 5%), lipopolysacchride (33% 6 9%), and Poly
I:C (25% 6 5%), but not with peptidoglycan or CpG oligonucleotides.
ACs displayed a similar level of activation to
these treatments, and in addition responded to peptidoglycan.
The activation of OECs and ACs was enhanced by coculture
with MG (56% 6 16% and 85% 6 13%, respectively).
In contrast, SCs did not respond to any treatment
or to costimulation by MG. Immunostaining for the chemokine
Gro demonstrated a functional response that was consistent
with NFkB activation. OECs expressed mRNA for
Toll-like receptors (TLRs) 2 and 4, but only TLR4 protein
was detected by Western blotting and immunohistochemistry.
The results demonstrate that OECs possess the cellular
machinery that permits them to respond to certain bacterial
ligands, and may have an innate immune function in
protecting the CNS against infection.

Item Type: Article
Keywords: glia; cytokines; Toll-like receptors; neuroimmunomodulation; innate immune system
Journal or Publication Title: Glia
Publisher: Wiley-Liss
Page Range: pp. 905-916
ISSN: 0894-1491
Identification Number - DOI: 10.1002/glia.20512
Additional Information:

The original publication is available at
http://www.interscience.wiley.com/

Date Deposited: 07 Apr 2008 14:40
Last Modified: 18 Nov 2014 03:35
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