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Transplantation of 3D adipose‑derived stem cell/hepatocyte spheroids alleviates chronic hepatic damage in a rat model of thioacetamide‑induced liver cirrhosis

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Wu, YC, Wu, GX, Chen, KW, Shiu, L-Y, Kumar, S, Liu, G-S ORCID: 0000-0003-3379-724X and Luo, SM 2022 , 'Transplantation of 3D adipose‑derived stem cell/hepatocyte spheroids alleviates chronic hepatic damage in a rat model of thioacetamide‑induced liver cirrhosis' , Scientific Reports, vol. 12 , pp. 1-9 , doi: 10.1038/s41598-022-05174-2.

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Abstract

Cirrhosis refers to irreversible liver damage where healthy tissue is replaced by scar tissue, resulting in impaired liver function. There is no cure and current treatments only prevent further liver damage; thus, novel therapeutic options are urgently needed. Here, we report a new approach that enables the formation of self-assembled 3D spheroids of adipose-derived stem cells (ADSCs) and murine hepatocytes (AML12) via reconstituted collagen fibers. Compared with the spheroids formed in the commercially available EZSHERE dish, the collagen fiber-based ADSC/hepatocyte spheroids offer a notable benefit in structure formation and paracrine factor secretion. To test the regenerative capability of the collagen fiber-based 3D ADSC/hepatocyte spheroids, a rat model of thioacetamide (TAA)-induced liver cirrhosis was employed. The transplantation of the collagen fiber-based 3D ADSC/hepatocyte spheroids show an improvement in liver function and ameliorates pathological liver cirrhosis in TAA-treated rats. In summary, our data show collagen fiber-based self-assembled 3D ADSC/hepatocyte spheroids to possess the excellent regenerative capacity in response to TAA-induced liver injury, promising an alternative therapeutic strategy for liver cirrhosis.

Item Type: Article
Authors/Creators:Wu, YC and Wu, GX and Chen, KW and Shiu, L-Y and Kumar, S and Liu, G-S and Luo, SM
Keywords: stem cell, 3D cell spheroids, liver cirrhosis
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
DOI / ID Number: 10.1038/s41598-022-05174-2
Copyright Information:

© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. http://creat ivecommons.org/licenses/by/4.0/.

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