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Characterization and genomic analysis of the first podophage infecting Shewanella, representing a novel viral cluster

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Dong, Y, Zheng, K, Zou, X, Liang, Y, Liu, Y, Li, X, Shao, H, Sung, YY, Mok, WJ, Wong, LL, McMinn, A ORCID: 0000-0002-2133-3854 and Wang, M 2022 , 'Characterization and genomic analysis of the first podophage infecting Shewanella, representing a novel viral cluster' , Frontiers in Microbiology, vol. 13 , pp. 1-13 , doi: 10.3389/fmicb.2022.853973.

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Abstract

Shewanella is a common bacterial genus in marine sediments and deep seas, with a variety of metabolic abilities, suggesting its important roles in the marine biogeochemical cycles. In this study, a novel lytic Shewanella phage, vB_SInP-X14, was isolated from the surface coastal waters of Qingdao, China. The vB_SInP-X14 contains a linear, double-strand 36,396-bp with the G + C content of 44.1% and harbors 40 predicted open reading frames. Morphological, growth, and genomic analysis showed that it is the first isolated podovirus infecting Shewanella, with a short propagation time (40 min), which might be resulted from three lytic-related genes. Phylogenetic analysis suggested that vB_SInP-X14 could represent a novel viral genus, named Bocovirus, with four isolated but not classified phages. In addition, 14 uncultured viral genomes assembled from the marine metagenomes could provide additional support to establish this novel viral genus. This study reports the first podovirus infecting Shewanella, establishes a new interaction system for the study of virus–host interactions, and also provides new reference genomes for the marine viral metagenomic analysis.

Item Type: Article
Authors/Creators:Dong, Y and Zheng, K and Zou, X and Liang, Y and Liu, Y and Li, X and Shao, H and Sung, YY and Mok, WJ and Wong, LL and McMinn, A and Wang, M
Keywords: Shewanella, podophage, genomic analysis, phylogenetic analysis, novel viral cluster
Journal or Publication Title: Frontiers in Microbiology
Publisher: Frontiers Research Foundation
ISSN: 1664-302X
DOI / ID Number: 10.3389/fmicb.2022.853973
Copyright Information:

Copyright © 2022 Dong, Zheng, Zou, Liang, Liu, Li, Shao, Sung, Mok, Wong,McMinn and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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