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The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock

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Bommana, S, Jelocnik, M, Borel, N, Marsh, I, Carver, S ORCID: 0000-0002-3579-7588 and Polkinghorne, A 2019 , 'The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock' , Journal of Medical Microbiology, vol. 68, no. 4 , pp. 627-632 , doi: 10.1099/jmm.0.000951.

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Abstract

Chlamydia pecorumand Chlamydia abortus are related ruminant pathogens endemic to different global regions. Potential co-infections combined with the lack of species-specific serological assays challenge accurate diagnosis. Serological screening revealed low C. abortus seropositivity with the peptide-based ELISA (1/84; 1.2%) in Australian sheep yet moderate seropositivity in a Swiss flock with history of C. abortus-associated abortions (17/63; 26.9%). By whole cell antigen complement fixation tests (CFT) and ELISA, chlamydial seropositivity was significantly higher in all groups, suggesting cross-reactivity between these two chlamydial species and non-specificity of the tests. However, only C. pecorum DNA could be detected by qPCR in Chlamydia seropositive Australian animals screened, suggesting chlamydial seropositivity was due to cross-reactivity with endemic C. pecorum infections. These results suggest ascribing Chlamydia seropositivity to chlamydial species in livestock using whole-cell antigen CFT or ELISA should be treated with caution; and that peptide-based ELISA and qPCR provide greater chlamydial species-specificity.

Item Type: Article
Authors/Creators:Bommana, S and Jelocnik, M and Borel, N and Marsh, I and Carver, S and Polkinghorne, A
Keywords: Chlamydia abortus , Chlamydia pecorum , ovine enzootic abortion, serology, whole antigen based ELISA, peptide antigen based ELISA, epidemiology
Journal or Publication Title: Journal of Medical Microbiology
Publisher: The Microbiology Society
ISSN: 0022-2615
DOI / ID Number: 10.1099/jmm.0.000951
Copyright Information:

Copyright 2019 The Authors

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