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Lipid kinases PIP5K7 and PIP5K9 are required for polyamine-triggered K+ efflux in Arabidopsis roots

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Zarza, X, Van Wijk, R, Shabala, L ORCID: 0000-0002-5360-8496, Hunkeler, A, Lefebvre, M, Rodriguez-Villalon, A, Shabala, S ORCID: 0000-0003-2345-8981, Tiburcio, AF, Heilmann, I and Munnik, T 2020 , 'Lipid kinases PIP5K7 and PIP5K9 are required for polyamine-triggered K+ efflux in Arabidopsis roots' , The Plant Journal, vol. 104, no. 2 , pp. 416-432 , doi: 10.1111/tpj.14932.

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Abstract

Polyamines, such as putrescine, spermidine and spermine (Spm), are low-molecular-weight polycationic molecules present in all living organisms. Despite their implication in plant cellular processes, little is known about their molecular mode of action. Here, we demonstrate that polyamines trigger a rapid increase in the regulatory membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), and that this increase is required for polyamine effects on K+ efflux in Arabidopsis roots. Using in vivo 32Pi-labelling of Arabidopsis seedlings, low physiological (μm) concentrations of Spm were found to promote a rapid PIP2 increase in roots that was time- and dose-dependent. Confocal imaging of a genetically encoded PIP2 biosensor revealed that this increase was triggered at the plasma membrane. Differential 32Pi-labelling suggested that the increase in PIP2 was generated through activation of phosphatidylinositol 4-phosphate 5-kinase (PIP5K) activity rather than inhibition of a phospholipase C or PIP2 5-phosphatase activity. Systematic analysis of transfer DNA insertion mutants identified PIP5K7 and PIP5K9 as the main candidates involved in the Spm-induced PIP2 response. Using non-invasive microelectrode ion flux estimation, we discovered that the Spm-triggered K+ efflux response was strongly reduced in pip5k7 pip5k9 seedlings. Together, our results provide biochemical and genetic evidence for a physiological role of PIP2 in polyamine-mediated signalling controlling K+ flux in plants.

Item Type: Article
Authors/Creators:Zarza, X and Van Wijk, R and Shabala, L and Hunkeler, A and Lefebvre, M and Rodriguez-Villalon, A and Shabala, S and Tiburcio, AF and Heilmann, I and Munnik, T
Keywords: Arabidopsis, roots, K+ flux, polyamine, lipid kinases, PIP5K7, PIP5K9, phosphoinositide signalling, phosphatidylinositol 4,5-bisphosphate (PIP2), phosphatidylinositol 4-phosphate 5-kinase (PIP5K), phosphatidic acid (PA), phospholipids
Journal or Publication Title: The Plant Journal
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 1365-313X
DOI / ID Number: 10.1111/tpj.14932
Copyright Information:

© 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, (https://creativecommons.org/licenses/by/4.0/) which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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