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Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle

Lo, HP, Lim, Y-W, Xiong, Z, Martel, N, Ferguson, C, Ariotti, N, Giacomotto, J, Rae, J, Floetenmeyer, M, Moradi, SV, Gao, Y, Tillu, VA, Xia, D, Wang, H, Rahnama, S, Nixon, SJ, Bastiani, M, Day, RD ORCID: 0000-0002-1834-6945, Smith, KA, Palpant, NJ, Johnston, WA, Alexandrov, K, Collins, BM, Hall, TE and Parton, RG 2021 , 'Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle' , Journal of Cell Biology, vol. 220, no. 12 , pp. 1-21 , doi:

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The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems, mouse and zebrafish. In both models, Cavin4 localized to T-tubules, and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicated cavin4 paralogs, Cavin4b was shown to directly interact with the T-tubule-associated BAR domain protein Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+ response upon mechanical stimulation. We propose a role for Cavin4 in remodeling the T-tubule membrane early in development by recycling caveolar components from the T-tubule to the sarcolemma. This generates a stable T-tubule domain lacking caveolae that is essential for T-tubule function.

Item Type: Article
Authors/Creators:Lo, HP and Lim, Y-W and Xiong, Z and Martel, N and Ferguson, C and Ariotti, N and Giacomotto, J and Rae, J and Floetenmeyer, M and Moradi, SV and Gao, Y and Tillu, VA and Xia, D and Wang, H and Rahnama, S and Nixon, SJ and Bastiani, M and Day, RD and Smith, KA and Palpant, NJ and Johnston, WA and Alexandrov, K and Collins, BM and Hall, TE and Parton, RG
Keywords: skeletal muscle, cavin proteins, Cavin4
Journal or Publication Title: Journal of Cell Biology
Publisher: Rockefeller Univ Press
ISSN: 0021-9525
DOI / ID Number:
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© 2021 Lo et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at

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