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Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle

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Lo, HP, Lim, Y-W, Xiong, Z, Martel, N, Ferguson, C, Ariotti, N, Giacomotto, J, Rae, J, Floetenmeyer, M, Moradi, SV, Gao, Y, Tillu, VA, Xia, D, Wang, H, Rahnama, S, Nixon, SJ, Bastiani, M, Day, RD ORCID: 0000-0002-1834-6945, Smith, KA, Palpant, NJ, Johnston, WA, Alexandrov, K, Collins, BM, Hall, TE and Parton, RG 2021 , 'Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle' , Journal of Cell Biology, vol. 220, no. 12 , pp. 1-21 , doi: https://doi.org/10.1083/jcb.201905065.

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Abstract

The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems, mouse and zebrafish. In both models, Cavin4 localized to T-tubules, and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicated cavin4 paralogs, Cavin4b was shown to directly interact with the T-tubule-associated BAR domain protein Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+ response upon mechanical stimulation. We propose a role for Cavin4 in remodeling the T-tubule membrane early in development by recycling caveolar components from the T-tubule to the sarcolemma. This generates a stable T-tubule domain lacking caveolae that is essential for T-tubule function.

Item Type: Article
Authors/Creators:Lo, HP and Lim, Y-W and Xiong, Z and Martel, N and Ferguson, C and Ariotti, N and Giacomotto, J and Rae, J and Floetenmeyer, M and Moradi, SV and Gao, Y and Tillu, VA and Xia, D and Wang, H and Rahnama, S and Nixon, SJ and Bastiani, M and Day, RD and Smith, KA and Palpant, NJ and Johnston, WA and Alexandrov, K and Collins, BM and Hall, TE and Parton, RG
Keywords: skeletal muscle, cavin proteins, Cavin4
Journal or Publication Title: Journal of Cell Biology
Publisher: Rockefeller Univ Press
ISSN: 0021-9525
DOI / ID Number: https://doi.org/10.1083/jcb.201905065
Copyright Information:

© 2021 Lo et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
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