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Association between canine leishmaniosis and Ehrlichia canis co-infection: a prospective case-control study

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Attipa, C, Solano-Gallego, L, Papasouliotis, K, Soutter, F, Morris, D, Helps, C, Carver, SS ORCID: 0000-0002-3579-7588 and Tasker, S 2018 , 'Association between canine leishmaniosis and Ehrlichia canis co-infection: a prospective case-control study' , Parasites & Vectors, vol. 11 , p. 184 , doi: 10.1186/s13071-018-2717-8.

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Abstract

BackgroundIn the Mediterranean basin, Leishmania infantum is a major cause of disease in dogs, which are frequently co-infected with other vector-borne pathogens (VBP). However, the associations between dogs with clinical leishmaniosis (ClinL) and VBP co-infections have not been studied. We assessed the risk of VBP infections in dogs with ClinL and healthy controls.MethodsWe conducted a prospective case-control study of dogs with ClinL (positive qPCR and ELISA antibody for L. infantum on peripheral blood) and clinically healthy, ideally breed-, sex- and age-matched, control dogs (negative qPCR and ELISA antibody for L. infantum on peripheral blood) from Paphos, Cyprus. We obtained demographic data and all dogs underwent PCR on EDTA-blood extracted DNA for haemoplasma species, Ehrlichia/Anaplasma spp., Babesia spp., and Hepatozoon spp., with DNA sequencing to identify infecting species. We used logistic regression analysis and structural equation modelling (SEM) to evaluate the risk of VBP infections between ClinL cases and controls.ResultsFrom the 50 enrolled dogs with ClinL, DNA was detected in 24 (48%) for Hepatozoon spp., 14 (28%) for Mycoplasma haemocanis, 6 (12%) for Ehrlichia canis and 2 (4%) for Anaplasma platys. In the 92 enrolled control dogs, DNA was detected in 41 (45%) for Hepatozoon spp., 18 (20%) for M. haemocanis, 1 (1%) for E. canis and 3 (3%) for A. platys. No Babesia spp. or “Candidatus Mycoplasma haematoparvum” DNA was detected in any dog. No statistical differences were found between the ClinL and controls regarding age, sex, breed, lifestyle and use of ectoparasitic prevention. A significant association between ClinL and E. canis infection (OR = 12.4, 95% CI: 1.5–106.0, P = 0.022) was found compared to controls by multivariate logistic regression. This association was confirmed using SEM, which further identified that younger dogs were more likely to be infected with each of Hepatozoon spp. and M. haemocanis, and dogs with Hepatozoon spp. were more likely to be co-infected with M. haemocanis.ConclusionsDogs with ClinL are at a higher risk of co-infection with E. canis than clinically healthy dogs. We recommend that dogs diagnosed with ClinL should be tested for E. canis co-infection using PCR.

Item Type: Article
Authors/Creators:Attipa, C and Solano-Gallego, L and Papasouliotis, K and Soutter, F and Morris, D and Helps, C and Carver, SS and Tasker, S
Keywords: epidemiology, canine leishmaniosis, Leishmania infantum, Ehrlichia canis, vector-borne pathogen, co-infection, Cyprus, Anaplasma platys, Mycoplasma haemocanis, Hepatozoon spp., structural equation model
Journal or Publication Title: Parasites & Vectors
Publisher: BioMed Central Ltd.
ISSN: 1756-3305
DOI / ID Number: 10.1186/s13071-018-2717-8
Copyright Information:

© 2018. The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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