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Abstract

Background/Aim: The lack of specific parathyroid carcinoma (PC) biomarkers in clinical practice points out the importance of analyzing the proteomic signature of this cancer. We performed a comparative proteomic analysis of PC and parathyroid adenoma (PA) co-existing in the same patient. Patients and Methods: PC and PA were taken from a 63-year-old patient. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled to mass spectrometry we examined the differences between PC and PA proteins. For validation, additional PC and PA samples were obtained from 10 patients. Western blot analysis was used to validate the difference of expression observed with 2D-DIGE analysis. Bioinfomatic analysis was performed using QIAGENs Ingenuity Pathways Analysis (IPA) to determine the predominant canonical pathways and interaction networks involved. Results: Thirty-three differentially expressed proteins were identified in PC compared to PA. Among these, ubiquitin C-terminal hydrolase-L1 (UCH-L1) was highly overexpressed in PC. The result was confirmed by Western Blot analysis in additional PC samples. Conclusion: Our comparative proteomic analysis of co-existing neoplasms allowed detecting specific and peculiar differences between PC and PA overcoming population biological variability.

Item Type:	Article
Authors/Creators:	Ciregia, F and Cetani, F and Pardi, E and Soggiu, A and Piras, C and Zallocco, L and Borsari, S and Ronci, M and Caruso, V and Marcocci, C and Mazzoni, MR and Lucacchini, A and Giusti, L
Keywords:	proteomics, purinergic system, drug discovery
Journal or Publication Title:	Cancer Genomics and Proteomics
Publisher:	International Institute of Anticancer Research
ISSN:	1109-6535
DOI / ID Number:	https://doi.org/10.21873/cgp.20297
Copyright Information:	Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved
Related URLs:	Google Scholar: Caruso, V UTAS Profile: Caruso, V
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