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Changes in expression level of OsHKT1;5 alters activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in salinized rice roots

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Alnayef, M, Solis, C, Shabala, L ORCID: 0000-0002-5360-8496, Ogura, T, Chen, Z, Bose, J, Maathuis, FJM, Venkataraman, G, Tanoi, K, Yu, M, Zhou, M ORCID: 0000-0003-3009-7854, Horie, T and Shabala, S ORCID: 0000-0003-2345-8981 2020 , 'Changes in expression level of OsHKT1;5 alters activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in salinized rice roots' , International Journal of Molecular Sciences, vol. 21, no. 14 , doi: 10.3390/ijms21144882.

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Abstract

In rice, the OsHKT1;5 gene has been reported to be a critical determinant of salt tolerance. This gene is harbored by the SKC1 locus, and its role was attributed to Na+ unloading from the xylem. No direct evidence, however, was provided in previous studies. Also, the reported function of SKC1 on the loading and delivery of K+ to the shoot remains to be explained. In this work, we used an electrophysiological approach to compare the kinetics of Na+ uptake by root xylem parenchyma cells using wild type (WT) and NIL(SKC1) plants. Our data showed that Na+ reabsorption was observed in WT, but not NIL(SKC1) plants, thus questioning the functional role of HKT1;5 as a transporter operating in the direct Na+ removal from the xylem. Instead, changes in the expression level of HKT1;5 altered the activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in the rice epidermis and stele, explaining the observed phenotype. We conclude that the role of HKT1;5 in plant salinity tolerance cannot be attributed to merely reducing Na+ concentration in the xylem sap but triggers a complex feedback regulation of activities of other transporters involved in the maintenance of plant ionic homeostasis and signaling under stress conditions.

Item Type: Article
Authors/Creators:Alnayef, M and Solis, C and Shabala, L and Ogura, T and Chen, Z and Bose, J and Maathuis, FJM and Venkataraman, G and Tanoi, K and Yu, M and Zhou, M and Horie, T and Shabala, S
Keywords: rice roots, salinity, membrane transporters, OsHKT1;5, K+, Ca2+, salinity stress, xylem loading, sodium, potassium, SKC1, HAK5, GORK, RBOHD, epidermis, stele
Journal or Publication Title: International Journal of Molecular Sciences
Publisher: Molecular Diversity Preservation International
ISSN: 1422-0067
DOI / ID Number: 10.3390/ijms21144882
Copyright Information:

Copyright 2020 The Author(s)Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/

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