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The lncRNA PILA promotes NF-κB signaling in osteoarthritis by stimulating the activity of the protein arginine methyltransferase PRMT1

Tang, S, Cao, Y, Cai, Z, Nie, X, Ruan, J, Zhou, Z, Ruan, G, Zhu, Z, Han, W and Ding, C ORCID: 0000-0002-9479-730X 2022 , 'The lncRNA PILA promotes NF-κB signaling in osteoarthritis by stimulating the activity of the protein arginine methyltransferase PRMT1' , Science Signaling, vol. 15, no. 735 , pp. 1-15 , doi: 10.1126/scisignal.abm6265.

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Abstract

Inflammatory cytokineinduced activation of nuclear factor κB (NF-κB) signaling plays a critical role in the pathogenesis of osteoarthritis (OA). We identified PILA as a long noncoding RNA (lncRNA) that enhances NF-κB signaling and OA. The abundance of PILA was increased in damaged cartilage from patients with OA and in human articular chondrocytes stimulated with the proinflammatory cytokine tumor necrosis factor (TNF). Knockdown of PILA inhibited TNF-induced NF-κB signaling, extracellular matrix catabolism, and apoptosis in chondrocytes, whereas ectopic expression of PILA promoted NF-κB signaling and matrix degradation. PILA promoted PRMT1-mediated arginine methylation of DExH-box helicase 9 (DHX9), leading to an increase in the transcription of the gene encoding transforming growth factor βactivated kinase 1 (TAK1), an upstream activator of NF-κB signaling. Furthermore, intra-articular injection of an adenovirus vector encoding PILA triggered spontaneous cartilage loss and exacerbated posttraumatic OA in mice. This study provides insight into the regulation of NF-κB signaling in OA and identifies a potential therapeutic target for this disease.

Item Type: Article
Authors/Creators:Tang, S and Cao, Y and Cai, Z and Nie, X and Ruan, J and Zhou, Z and Ruan, G and Zhu, Z and Han, W and Ding, C
Keywords: cytokines, interleukin-1beta, NF-kappa B, long noncoding RNA, repressor, proteins, PRMT1 protein, human, Arginine N-Methyltransferases
Journal or Publication Title: Science Signaling
Publisher: American Association for the Advancement of Science (AAAS)
ISSN: 1937-9145
DOI / ID Number: 10.1126/scisignal.abm6265
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Copyright © 2022 The Author.

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