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A DNA barcode reference library for endemic Ponto‑Caspian amphipods

Copilas-Ciocianu, D, Rewicz, T, Sands, AF, Palatov, D, Marin, I, Arbaciauskas, K, Hebert, PDN, Grabowski, M and Audzijonyte, A ORCID: 0000-0002-9919-9376 2022 , 'A DNA barcode reference library for endemic Ponto‑Caspian amphipods' , Scientific Reports, vol. 12 , pp. 1-14 , doi:

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The Ponto-Caspian region is an endemicity hotspot that harbours several crustacean radiations, among which amphipods are the most diverse. These poorly known species are severely threatened in their native range, while at the same time they are invading European inland waters with significant ecological consequences. A proper taxonomic knowledge of this fauna is paramount for its conservation within the native region and monitoring outside of it. Here, we assemble a DNA barcode reference library for nearly 60% of all known Ponto-Caspian amphipod species. We use several methods to define molecular operational taxonomic units (MOTUs), based on two mitochondrial markers (COI and 16S), and assess their congruence with current species-level taxonomy based on morphology. Depending on the method, we find that 5469% of species had congruent morpho-molecular boundaries. The cases of incongruence resulted from lumping distinct morphospecies into a single MOTU (727%), splitting a morphospecies into several MOTUs (428%), or both (411%). MOTUs defined by distance-based methods without a priori divergence thresholds showed the highest congruence with morphological taxonomy. These results indicate that DNA barcoding is valuable for clarifying the diversity of Ponto-Caspian amphipods, but reveals that extensive work is needed to resolve taxonomic uncertainties. Our study advances the DNA barcode reference library for the European aquatic biota, paving the way towards improved taxonomic knowledge needed to enhance monitoring and conservation efforts.

Item Type: Article
Authors/Creators:Copilas-Ciocianu, D and Rewicz, T and Sands, AF and Palatov, D and Marin, I and Arbaciauskas, K and Hebert, PDN and Grabowski, M and Audzijonyte, A
Keywords: Ponto-Caspian, molecular systematics, species identification, amphipods, ancient lakes
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
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© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License, (, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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