Open Access Repository

Sodium dodecyl sulfate removal during electrospray ionization using cyclodextrins as simple sample solution additive for improved mass spectrometric detection of peptides

Quirino, JP ORCID: 0000-0002-8275-9672 2018 , 'Sodium dodecyl sulfate removal during electrospray ionization using cyclodextrins as simple sample solution additive for improved mass spectrometric detection of peptides' , Analytica Chimica Acta: International Journal Devoted to All Branches of Analytical Chemistry, vol. 1005 , pp. 54-60 , doi: 10.1016/j.aca.2017.12.012.

Full text not available from this repository.

Abstract

Sodium dodecyl sulfate (SDS) removal is a vital procedure in SDS-assisted bottom-up proteomics because SDS affects the quality of the data in electrospray ionization mass spectrometry (ESI-MS). SDS removal methods provide efficient removal of SDS and improved peptide analysis, but would usually require time, specialised devices, and experienced analysts. Here, by simple addition of γ-cyclodextrin (γ-CD) to the solution at concentrations 1 to 2x the SDS in the sample, the SDS related signals in positive ionization ESI-MS can be significantly removed (7099% reduction), without an additional sample manipulation step of extraction or purification. The mechanism for removal is based on the formation of tightly bound CD-SDS inclusion complexes, which hampered the generation of positively charged SDS multimers during ESI. For a sample with peptides (glu-val-phe, tyr-tyr-tyr, and bradykinin) and 3 mM SDS where 6 mM γ-CD was added, the %signal recoveries of peptides calculated by comparison with signals from standard samples without SDS were 4959%. The space charge effect by SDS on bradykinin was also reduced, increasing the signal for bradykinin 12x in the presence of γ-CD. For a protein (bovine serum albumin, BSA) digest with 3 mM SDS, which is an expected concentration in trypsin treated samples, a noticeable 7-fold improvement in the peptide to SDS signal ratio and a 91% reduction of SDS signals were observed upon addition of 6 mM γ-CD. However, there were only small changes in the ESI-MS intensities for the BSA peptides (compared to without addition of γ-CD). This new approach to SDS signal removal using CDs in ESI-MS may find use in proteomic studies.

Item Type: Article
Authors/Creators:Quirino, JP
Keywords: Electrospray ionization mass spectrometry, sodium dodecyl sulfate, cyclodextrin, inclusion complexes, sample matrix
Journal or Publication Title: Analytica Chimica Acta: International Journal Devoted to All Branches of Analytical Chemistry
Publisher: Elsevier Science Bv
ISSN: 0003-2670
DOI / ID Number: 10.1016/j.aca.2017.12.012
Copyright Information:

© 2018 Elsevier B.V. All rights reserved.

Related URLs:
Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page
TOP