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Abstract

Male specific DNA sequences were selected from a Diversity Arrays Technology (DArT) mapping study to evaluate their suitability for determination of the sex phenotype among young seedlings in a hop (Humulus lupulus L.) breeding program. Ten male specific DArT markers showed complete linkage with male sex phenotype in three crossing families. Following optimization, four were successfully converted into PCR markers and a multiplex PCR approach for their use was developed. Among 197 plants (97 from the world collection; 100 from three segregating families), 94-100% positive correlation with sex phenotypic data was achieved for the single PCR amplification, whereas the multiplex approach showed 100% correlation. To develop a fast and low-cost method, crude sample multiplex PCR was evaluated in 253 progenies from 14 segregating populations without losing accuracy. The study describes, for the first time, the routine application of molecular markers linked to male sex in an intensive Slovenian hop breeding program. The methods described could be employed for screening of sex at the seedling stage in other hop programs worldwide, thereby saving resources for desirable female plants.

Item Type:	Article
Authors/Creators:	Cerenak, A and Kolenc, Z and Sehur, P and Whittock, SP and Koutoulis, A and Beatson, R and Buck, E and Javornik, B and Skof, S and Jakse, J
Keywords:	Diversity Arrays Technology, genetic mapping, Humulus lupulus, hops, hop breeding
Journal or Publication Title:	Scientific Reports
Publisher:	Nature Publishing Group
ISSN:	2045-2322
DOI / ID Number:	https://doi.org/10.1038/s41598-019-50400-z
Copyright Information:	Copyright 2019 the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Related URLs:	UTAS Profile: Whittock, SP UTAS Profile: Koutoulis, A
Item Statistics:	View statistics for this item

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