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Use of bioaffinity interactions in electrokinetically controlled assays on microfabricated devices


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Guijt, RM and Baltussen, E and van Dedem, GWK (2002) Use of bioaffinity interactions in electrokinetically controlled assays on microfabricated devices. Electrophoresis, 23 (6). pp. 823-835. ISSN 0173-0835

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In this contribution, the role of bioaffinity interactions on electrokinetically controlled microfabricated devices is reviewed. Interesting applications reported in the literature include enzymatic assays, where enzyme and enzyme inhibition kinetics were studied, often in combination with electrophoretic separation. Attention is paid towards developments that could lead to implementation of electrokinetically controlled microdevices in high-throughput screening. Furthermore, enzyme-facilitated detection in combination with electrophoretic separation on microdevices is discussed. Various types of immunoassays have been implemented on the microchip format. The selectivity of antibody-antigen interaction has been exploited for the detection of analytes in complex sample matrices as required, for example, in clinical chemistry. Binding kinetics as well as stoichiometry were studied in chip-based assays. Automated mixing protocols as well as the demonstration of a parallel immunoassay allow implementation of microdevices in high-throughput screening. Furthermore, demonstration of immunoassays on cheap polymeric microdevices opens the way towards the fabrication of disposable devices, a requirement for commercialization and therefore for application in routine analyses.

Item Type: Article
Keywords: Affinity interactions; Binding constants; Electrophoresis; Molecular recognition; Review; -Total analysis system
Journal or Publication Title: Electrophoresis
Page Range: pp. 823-835
ISSN: 0173-0835
Identification Number - DOI: 10.1002/1522-2683(200203)23:6<823::AID-ELPS823>3.0.CO;2-Z
Additional Information:

Copyright 2002 Wiley-VCH Verlag GmbH & Co. KGaA

Date Deposited: 20 Apr 2008 12:04
Last Modified: 18 Nov 2014 03:38
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