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Capillary electrophoresis with on-chip four-electrode capacitively coupled conductivity detection for application in bioanalysis
journal contribution
posted on 2023-05-26, 15:22 authored by Rosanne Guijt, Baltussen, E, van der Steen, G, Frank, J, Billiet, HAH, Schalkhammer, T, Laugere, F, Vellekoop, MJ, Berthold, A, Sarro, PM, van Dedem, GWKMicrochip capillary electrophoresis (CE) with integrated four-electrode capacitively coupled conductivity detection is presented. Conductivity detection is a universal detection technique that is relatively independent on the detection pathlength and, especially important for chip-based analysis, is compatible with miniaturization and on-chip integration. The glass microchip structure consists of a 6 cm etched channel (20 ˜í¬¿m ‚àöv= 70 ˜í¬¿m cross section) with silicon nitride covered walls. In the channel, a 30 nm thick silicon carbide layer covers the electrodes to enable capacitive coupling with the liquid inside the channel as well as to prevent interference of the applied separation field. The detector response was found to be linear over the concentration range from 20 ˜í¬¿M up to 2 mM. Detection limits were at the low ˜í¬¿m level. Separation of two short peptides with a pl of respectively 5.38 and 4.87 at the 1 mM level demonstrates the applicability for biochemical analysis. At a relatively low separation field strength (50 V/cm) plate numbers in the order of 3500 were achieved. Results obtained with the microdevice compared well with those obtained in a bench scale CE instrument using UV detection under similar conditions.
History
Publication title
ElectrophoresisVolume
22Article number
12Number
12Pagination
2537-2541ISSN
0173-0835Publication status
- Published
Rights statement
Copyright 2001 Wiley-VCH Verlag GmbH & Co. KGaARepository Status
- Restricted
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