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Recent advances in affinity capillary electrophoresis

Guijt, RM, Frank, J, van Dedem, GWK and Baltussen, E 2000 , 'Recent advances in affinity capillary electrophoresis' , Electrophoresis, vol. 21, no. 18 , pp. 3905-3918 , doi:<3905::AID-ELPS3905>3.0.CO;2-4.

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Use of the specificity of (bio)interactions can effectively overcome the selectivity limitation faced in capillary electrophoresis (CE), and the resulting technique usually is referred to as affinity capillary electrophoresis (ACE). Despite the high selectivity of ACE, several important problems still need to be addressed. A major issue in all CE separations, including ACE, is the concentration detection limit. Using UV detection, this is usually in the order of 10 - 6 M whereas laser-induced fluorescence (LIF) detection can provide detection limits down to the sub-10 - 10 M range. However, a marked disadvantage of LIF is that labeling of the analytes is usually required, which might change the interaction behavior of the solutes under investigation. Additionally, labeling reactions at sub-10 - 10 M concentration levels are certainly not trivial and often difficult to perform quantitatively. Alternative and universal detection approaches, particularly mass spectrometric (MS) detection, look very promising but (A) CE-MS techniques are still far from routine application. Important future progress in sensitive detection strategies is likely to increase the use of ACE in the future.

Item Type: Article
Authors/Creators:Guijt, RM and Frank, J and van Dedem, GWK and Baltussen, E
Keywords: Affinity capillary electrophoresis • Binding constants • Review
Journal or Publication Title: Electrophoresis
ISSN: 0173-0835
DOI / ID Number:<3905::AID-ELPS3905>3.0.CO;2-4
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