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Modelling of migration behaviour of inorganic anions in ion-exchange capillary electrochromatography


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Breadmore, MC, Hilder, EF, Macka, M, Avdalovic, N and Haddad, PR 2001 , 'Modelling of migration behaviour of inorganic anions in ion-exchange capillary electrochromatography' , Electrophoresis, vol. 22, no. 3 , pp. 503-510 , doi: 10.1002/1522-2683(200102)22:3<503::AID-ELPS503>3.0.CO;2-S.

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A theoretical model to explain the observed mobility of inorganic anions in capillary electrochromatography (CEC) using ion-exchange (IE) stationary phases has been derived. The model divides contributions to the observed mobility of an analyte ion into capillary electrophoretic (CE) and IE components. The CE component includes the influence of varying the ionic strength of the background electrolyte on the electrophoretic mobility of the analyte, while the IE component accounts for the variation in retention of the analyte ion caused by changing the composition of the background electrolyte. The model was verified using a mixture of UV-absorbing inorganic ions in electrolytes of differing eluotropic strength in both packed and open-tubular CEC systems, with excellent agreement (r2 > 0.98) for both systems. Values of constants in the model equation determined by nonlinear regression were used to estimate the relative strengths of the interactions of different analytes with the stationary phase and these were found to agree well with elution orders observed in conventional IE chromatography.

Item Type: Article
Authors/Creators:Breadmore, MC and Hilder, EF and Macka, M and Avdalovic, N and Haddad, PR
Keywords: Ion exchange • Electrochromatography • Packed column • Open-tubular column • Modelling • Optimisation • Inorganic anions
Journal or Publication Title: Electrophoresis
ISSN: 0173-0835
DOI / ID Number: 10.1002/1522-2683(200102)22:3<503::AID-ELPS503>3.0.CO;2-S
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© 2001 WILEY-VCH Verlag GmbH

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